Electrochemical analyte detection apparatus and method

• US 9,939,440 B2
• Filed: 02/08/2016
• Issued: 04/10/2018
• Est. Priority Date: 07/26/2007
• Status: Active Grant

• Current Assignee: Agamatrix Incorporated (AgaMatrix Holdings LLC d/b/a WaveForm Diabetes)
• Original Assignee: Agamatrix Incorporated (AgaMatrix Holdings LLC d/b/a WaveForm Diabetes)
• Inventors: Harding, Ian, Iyengar, Sridhar, Williams, Richard
• Primary Examiner(s): Dinh, Bach T
• Application Number:
  US15/017,787
• Publication Number:
  US 20160231321A1
• Time in Patent Office: 792 Days
• Field of Search: 20440301-40315, 2057775-778
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 2333/90 Enzymes; Proenzymes
• CPC Class Codes: G01N 2333/902 Oxidoreductases (1.)
• CPC Class Codes: G01N 27/4166 measuring a particular property of an electrolyte
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes

Abstract

A method and apparatus for electrochemical detection of analyte in a sample makes use of a binding interaction and relies on the discovery that asymmetric distribution of a redox enzyme between two electrodes that occurs when a redox enzyme-containing reagent is immobilized at the surface of one electrode can be detected as a chemical potential gradient arising from an asymmetry in the distribution of oxidized or reduced redox substrate. This chemical potential gradient can be detected potentiometrically by observing the potential difference between the electrodes in an open circuit, or amperometrically by observing the current flow between the electrodes when the circuit is closed. In both cases, the observation of asymmetry can be done without the application of an external potential or current to the electrodes.

Claims

1. A method for determining the amount of enzyme present at one electrode independent of the amount of enzyme present at a second electrode, comprising the steps of:

(a) introducing a sample to a test cell comprising first and second electrodes, a mobile test reagent comprising a redox enzyme portion, and reagents that interact with the mobile test reagent and/or the analyte, such that the mobile test reagent has a first distribution relative to the electrodes when analyte is present in the sample and a second distribution relative to the electrodes when analyte is not present sample, one of said first and second distributions being asymmetric with respect to a line between the electrodes, and the other of the first and second distributions being symmetric or less asymmetric with respect to the line between the electrodes,

(b) supplying a redox substrate for the redox enzyme in the test cell, said redox substrate being acted upon by the redox enzyme to produce a chemical potential gradient between the first and second electrodes, wherein the magnitude of the chemical potential gradient is determined by the distribution of the mobile test reagent and thus on the presence of analyte,

(c) detecting the chemical potential gradient, I₀, between the first and second electrodes to determine the ratio of enzyme activity present at the two electrodes, wherein the formation and detection of the chemical potential gradient are performed without application of an external potential or current,

(d) applying a potential and measuring the resulting current, I₃₀₀, between the first and second electrodes, and

(e) calculating an amount of enzyme, E₁, present at one electrode.

2. The method of claim 1, wherein the test cell comprises as one of the reagents that interact with the analyte an immobilized test reagent disposed on the surface of the first electrode, said immobilized test reagent comprising an analyte binding portion, and the mobile test reagent comprises an analyte-binding portion and the redox enzyme portion, whereby if analyte is present in the sample in the test cell, at least a portion of the mobile test reagent becomes immobilized on the first electrode thereby creating an asymmetric distribution of redox enzyme concentration between the first and second electrodes when analyte is present.

3. The method of claim 2, wherein the analyte binding portion of the immobilized test reagent and the analyte are an antibody-antigen pair or antibody/hapten pair.

4. The method of claim 3, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

5. The method of claim 2, wherein the analyte binding portion of the immobilized test reagent is a drug receptor.

6. The method of claim 5, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

7. The method of claim 2, wherein the analyte binding portion of the immobilized test reagent is a hormone receptor.

8. The method of claim 7, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

9. The method of claim 1, wherein the analyte binding portion of the immobilized test reagent and the analyte are an antibody-antigen pair or antibody/hapten pair.

10. The method of claim 9, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

11. The method of claim 1 wherein the analyte binding portion of the immobilized test reagent is a drug receptor.

12. The method of claim 11, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

13. The method of claim 1, wherein the analyte binding portion of the immobilized test reagent is a hormone receptor.

14. The method of claim 13, wherein the redox enzyme and the redox substrate comprises glucose and a mediator compound.

15. The method of claim 1, wherein E1=(R×

E_t+R), where R is the ratio of enzyme activity present at the two electrodes, E_t is the total enzyme activity.