Methods and compositions for analyzing proteins
First Claim
Patent Images
1. A compound of the formula:
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T-L-M-D,wherein;
T comprises an antibody or a fragment thereof specific for a target protein or polypeptide;
L is an oxidation-labile linking group comprising an olefin, wherein the olefin comprises a vinyl sulfide, a vinyl ether, or an enamine;
M is a direct bond, or is a divalent moiety having up to 100 non-hydrogen atoms selected from the group consisting of carbon, nitrogen, oxygen, phosphorus, boron, and sulfur; and
D is a fluorescent dye, a fluorescent label, a chromogenic dye, a chromogenic label, or an electrochemical label.
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Abstract
Compositions, kits, and methods are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. The compositions can include a first reagent including a cleavage-inducing moiety and a first binding agent for a binding site that includes a post-translational modification of a target polypeptide.
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Citations
12 Claims
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1. A compound of the formula:
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T-L-M-D,wherein; T comprises an antibody or a fragment thereof specific for a target protein or polypeptide; L is an oxidation-labile linking group comprising an olefin, wherein the olefin comprises a vinyl sulfide, a vinyl ether, or an enamine; M is a direct bond, or is a divalent moiety having up to 100 non-hydrogen atoms selected from the group consisting of carbon, nitrogen, oxygen, phosphorus, boron, and sulfur; and D is a fluorescent dye, a fluorescent label, a chromogenic dye, a chromogenic label, or an electrochemical label. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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9. The compound of claim 1, wherein D is a fluorescent dye.
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10. The compound of claim 9, wherein D is a water-soluble rhodamine dye, a fluorescein, a 4,7-dichlorofluorescein, or a benzoxanthene dye.
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11. The compound of claim 10, wherein D is a fluorescein, where the fluorescein is selected from the group consisting of:
- 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2′
,7′
-dimethoxy-5- and 6-carboxy-4,7-dichlorofluorescein, 2′
,7′
-dimethoxy-4′
,5′
-dichloro-5- and 6-carboxyfluorescein, 2′
,7′
-dimethoxy-4′
,5′
-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 1′
,2′
,7′
,8′
-dibenzo-5- and 6-carboxy-4,7-dichlorofluorescein, 1′
,2′
,7′
,8′
-dibenzo-4′
,5′
-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 2′
,7′
-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, and 2′
,4′
,5′
,7′
-tetrachloro-5- and 6-carboxy-4,7-dichlorofluorescein.
- 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2′
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12. The compound of claim 10, wherein D is
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Specification
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Current AssigneeMonogram Biosciences, Inc. (Laboratory Corporation Of America Holdings)
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Original AssigneeMonogram Biosciences, Inc. (Laboratory Corporation Of America Holdings)
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InventorsSingh, Sharat, Salimi-Moosavi, Hossein, Tahir, Syed Hasan, Wallweber, Gerald J., Kirakossian, Hrair, Matray, Tracy J., Hernandez, Vincent S.
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Primary Examiner(s)Haq, Shafiqul
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Application NumberUS14/826,573Publication NumberTime in Patent Office970 DaysField of SearchUS Class CurrentCPC Class CodesC07K 16/00 Immunoglobulins [IGs], e.g....C07K 2317/40 characterized by post-trans...G01N 33/582 with fluorescent labelG01N 33/6803 General methods of protein ...G01N 33/6842 Proteomic analysis of subse...
