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Methods and compositions for analyzing proteins

  • US 9,939,447 B2
  • Filed: 08/14/2015
  • Issued: 04/10/2018
  • Est. Priority Date: 05/21/2001
  • Status: Expired due to Term
First Claim
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1. A compound of the formula:


  • T-L-M-D,wherein;

    T comprises an antibody or a fragment thereof specific for a target protein or polypeptide;

    L is an oxidation-labile linking group comprising an olefin, wherein the olefin comprises a vinyl sulfide, a vinyl ether, or an enamine;

    M is a direct bond, or is a divalent moiety having up to 100 non-hydrogen atoms selected from the group consisting of carbon, nitrogen, oxygen, phosphorus, boron, and sulfur; and

    D is a fluorescent dye, a fluorescent label, a chromogenic dye, a chromogenic label, or an electrochemical label.

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