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Biomatrix scaffolds for industrial scale dispersal

  • US 9,944,896 B2
  • Filed: 10/13/2015
  • Issued: 04/17/2018
  • Est. Priority Date: 07/02/2010
  • Status: Active Grant
First Claim
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1. A plurality of biomatrix particles produced according to a method comprising:

  • comprising;

    (a) perfusing or homogenizing the biological tissue with a buffer comprising a salt concentration from about 3.5 M NaCl to about 4.5 M NaCl;

    (b) perfusing the biological tissue or extracting the homogenate of step (a) with a delipidating buffer comprising lipases and detergents in a serum-free first medium, having a neutral pH and an osmolality from about 250 mOsm/kg to about 350 mOsm/kg, wherein the detergents do not comprise sodium dodecyl sulfate or Triton X-100;

    (c) perfusing the biological tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration ranging from about 2.0 M NaCl to about 5.0 M NaCl, the concentration chosen to not lose insoluble collagens identified in the biological tissue;

    (d) perfusing the biological tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer;

    (e) rinsing the biological tissue or homogenate of step (d) with a second medium having a neutral pH, is serum-free and having an osomolality ranging from about 250 mOsm/kg to about 350 mOsm/kg, thereby producing an intact or homogenized biomatrix scaffold from the biological tissue;

    (f) pulverizing the intact or homogenized biomatrix scaffold into a plurality of biomatrix particles ranging in size from about 1 nm to about 100 μ

    m.

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