High density DNA array
First Claim
1. A high density DNA array comprisinga patterned surface, said surface comprising a pattern of DNA binding regions separated by a non-DNA binding surface, andmore than 105 different DNAs comprising genomic sequences bound on the array at the DNA binding regions,wherein the DNA binding regions comprise oligonucleotides for binding the DNAs within the DNA binding regions and all of the DNA binding regions of the array comprise the same oligonucleotides, andwherein:
- the density of the DNA binding regions on the array is more than 100,000 per mm2,more than 50% of the DNA binding regions in the array have multiple copies of one single DNA of said more than 105 different DNAs,the sequence of the single DNA at each DNA binding region is not known, andthe array is not a bead array.
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Accused Products
Abstract
A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.
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Citations
13 Claims
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1. A high density DNA array comprising
a patterned surface, said surface comprising a pattern of DNA binding regions separated by a non-DNA binding surface, and more than 105 different DNAs comprising genomic sequences bound on the array at the DNA binding regions, wherein the DNA binding regions comprise oligonucleotides for binding the DNAs within the DNA binding regions and all of the DNA binding regions of the array comprise the same oligonucleotides, and wherein: -
the density of the DNA binding regions on the array is more than 100,000 per mm2, more than 50% of the DNA binding regions in the array have multiple copies of one single DNA of said more than 105 different DNAs, the sequence of the single DNA at each DNA binding region is not known, and the array is not a bead array. - View Dependent Claims (2, 3, 4, 5)
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6. A high density DNA array for sequencing genomic DNA sequences, said array comprising:
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a patterned surface, said surface comprising a pattern of DNA binding regions at a density of more than 105 DNA binding regions per mm2, separated by non-DNA binding regions of said surface, wherein more than 50% of the DNA binding regions on said surface comprise multiple copies of a single genomic DNA sequence, wherein the DNA binding regions on said surface comprise more than 105 different genomic DNA sequences, wherein the genomic DNA sequences are randomly disposed on the array such that the genomic sequence at any given DNA binding region is not known prior to sequencing, the more than 105 different genomic DNA sequences are linked to adaptor sequences, the DNA binding regions comprise DNA capture oligonucleotides attached in the DNA binding regions and not attached to the non-DNA binding surface, all of the DNA binding regions on the surface comprise the same attached capture oligonucleotides, the capture oligonucleotides are complementary to the adaptors, and wherein the array is not a bead array. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13)
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Specification