Integrated device for temporal binning of received photons

• US 9,945,779 B2
• Filed: 07/21/2017
• Issued: 04/17/2018
• Est. Priority Date: 08/08/2014
• Status: Active Grant

• Current Assignee: Quantum-Si Incorporated
• Original Assignee: Quantum-Si Incorporated
• Inventors: Rothberg, Jonathan M., Fife, Keith G., Boisvert, David
• Primary Examiner(s): LUU, THANH X
• Application Number:
  US15/656,139
• Publication Number:
  US 20170322153A1
• Time in Patent Office: 270 Days
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/0697 Pulsed lasers
• CPC Class Codes: G01S 17/894 3D imaging with simultaneous measurement of time-of-flight at a 2D array of receiver pixels, e.g. time-of-flight cameras or flash lidar
• CPC Class Codes: G01S 7/4863 Detector arrays, e.g. charge-transfer gates
• CPC Class Codes: G01S 7/4865 Time delay measurement, e.g. time-of-flight measurement, time of arrival measurement or determining the exact position of a peak peak detection in noise, signal conditioning G01S7/487
• CPC Class Codes: H01L 27/14603 Special geometry or disposition of pixel-elements, address-lines or gate-electrodes
• CPC Class Codes: H01L 27/14687 Wafer level processing
• CPC Class Codes: H01L 27/14812 Special geometry or disposition of pixel-elements, address lines or gate-electrodes
• CPC Class Codes: H04N 25/77 Pixel circuitry, e.g. memories, A/D converters, pixel amplifiers, shared circuits or shared components
• CPC Class Codes: H04N 25/771 comprising storage means other than floating diffusion
• CPC Class Codes: H04N 25/772 comprising A/D, V/T, V/F, I/T or I/F converters

Abstract

An integrated circuit includes a photodetection region configured to receive incident photons. The photodetection region is configured to produce a plurality of charge carriers in response to the incident photons. The integrated circuit also includes at least one charge carrier storage region. The integrated circuit also includes a charge carrier segregation structure configured to selectively direct charge carriers of the plurality of charge carriers into the at least one charge carrier storage region based upon times at which the charge carriers are produced.

Claims

1. An integrated circuit, comprising:

a photodetection region;

at least one charge carrier storage region; and

a charge carrier segregation structure configured to;

remove, from the photodetection region, a first plurality of charge carriers generated therein by a first excitation light pulse, and discard the first plurality of charge carriers by preventing the first plurality of charge carriers from being stored in the at least one charge carrier storage region; and

following the removal of the first plurality of charge carriers generated by the first excitation light pulse, remove, from the photodetection region, a sensed charge carrier generated therein by a sensed photon, and direct the sensed charge carrier into the at least one charge carrier storage region prior to the photodetection region generating a second plurality of charge carriers by a second excitation light pulse,

wherein the first excitation light pulse excites a luminescent molecule, and wherein the sensed charge carrier is generated in the photodetection region by a sensed photon received from the luminescent molecule in response to the luminescent molecule being excited by the first excitation light pulse.

2. The integrated circuit of claim 1, wherein the charge carrier segregation structure is configured to direct the sensed charge carrier into a selected charge carrier storage region of the at least one charge carrier storage region based upon a time at which the sensed charge carrier is generated in the photodetection region.

3. The integrated circuit of claim 2, wherein the at least one charge carrier storage region comprises a plurality of charge carrier storage regions, and wherein the charge carrier segregation structure is configured to selectively direct sensed charge carriers into respective charge carrier storage regions of the plurality of charge carrier storage regions based on times which the sensed charge carriers are generated in the photodetection region.

4. The integrated circuit of claim 1, wherein the integrated circuit comprises a plurality of pixels, wherein a first pixel of the plurality of pixels comprises:

the photodetection region;

the at least one charge carrier storage region; and

the charge carrier segregation structure.

5. The integrated circuit of claim 4, wherein a second pixel of the plurality of pixels comprises:

a second photodetection region;

at least one second charge carrier storage region; and

a second charge carrier segregation structure.

6. The integrated circuit of claim 1, further comprising:

a control circuit configured to control the charge carrier segregation structure to perform a measurement, the measurement comprising;

a charge carrier capture phase in which the charge carrier segregation structure forms at least one potential barrier; and

after the charge carrier capture phase, a charge carrier transfer phase in which a sensed charge carrier, if captured during the charge carrier capture phase, is transferred to the at least one charge carrier storage region.

7. The integrated circuit of claim 6, wherein the control circuit is configured to control the charge carrier segregation structure to perform the measurement a plurality of times to aggregate sensed charge carriers in the at least one charge carrier storage region.

8. The integrated circuit of claim 7, wherein the plurality of times is at least one thousand times.

9. The integrated circuit of claim 8, wherein the plurality of times is at least one million times.

10. The integrated circuit of claim 9, wherein the plurality of measurements is performed in less than 50 milliseconds.

11. The integrated circuit of claim 10, wherein the plurality of measurements is performed in greater than one millisecond.

12. The integrated circuit of claim 6, wherein the control circuit is configured to perform the measurement based on a timing of the first excitation light pulse.

13. The integrated circuit of claim 1, wherein the integrated circuit is configured to:

(i) analyze one or more signals from the at least one charge carrier storage region to produce information regarding arrival of photons over time;

(ii) transmit information regarding one or more signals from the at least one charge carrier storage region to a computing device for analysis;

or

perform both (i) and (ii).

14. The integrated circuit of claim 13, wherein the integrated circuit or the computing device is configured to analyze the one or more signals to calculate a luminance lifetime or discriminate a first luminance lifetime from a second luminance lifetime.

15. The integrated circuit of claim 14, wherein the integrated circuit or the computing device is configured to analyze the one or more signals to calculate a fluorescence lifetime or discriminate a first fluorescence lifetime from a second fluorescence lifetime.

16. The integrated circuit of claim 1, wherein the integrated circuit is configured to detect the photons to sequence a nucleic acid.

17. The integrated circuit of claim 1, wherein the integrated circuit is configured to receive the photons from fluorophores including the luminescent molecule.

18. An integrated circuit, comprising:

a photodetection region;

at least one charge carrier storage region; and

means for;

removing, from the photodetection region, a first plurality of charge carriers generated therein by a first excitation light pulse, and discarding the first plurality of charge carriers by preventing the first plurality of charge carriers from being stored in the at least one charge carrier storage region; and

following the removal of the first plurality of charge carriers generated by the first excitation light pulse, removing, from the photodetection region, a sensed charge carrier generated therein by a sensed photon, and directing the sensed charge carrier into the at least one charge carrier storage region prior to the photodetection region generating a second plurality of charge carriers by a second excitation light pulse,

wherein the first excitation light pulse excites a luminescent molecule, and wherein the sensed charge carrier is generated in the photodetection region by a sensed photon received from the luminescent molecule in response to the luminescent molecule being excited by the first excitation light pulse.

19. A photodetection method, comprising:

receiving at a photodetection region, a first plurality of incident photons from a first excitation light pulse;

removing, from the photodetection region, a first plurality of charge carriers generated therein by the first excitation light pulse, and discarding the first plurality of charge carriers by preventing the first plurality of charge carriers from being stored in at least one charge carrier storage region; and

following the removal of the first plurality of charge carriers generated by the first excitation light pulse, removing, from the photodetection region, a sensed charge carrier generated therein by a sensed photon, and directing the sensed charge carrier into the at least one charge carrier storage region prior to the photodetection region generating a second plurality of charge carriers by a second excitation light pulse,

wherein the first excitation light pulse excites a luminescent molecule, and wherein the sensed charge carrier is generated in the photodetection region by a sensed photon received from the luminescent molecule in response to the luminescent molecule being excited by the first excitation light pulse.