Microfluidic device for the manipulation of particles

• US 9,950,322 B2
• Filed: 12/22/2011
• Issued: 04/24/2018
• Est. Priority Date: 12/22/2010
• Status: Active Grant

• Current Assignee: Menarini Silicon Biosystems S.P.A
• Original Assignee: Menarini Silicon Biosystems S.P.A
• Inventors: Medoro, Gianni, Manaresi, Nicol
• Primary Examiner(s): Alexander, Lyle
• Assistant Examiner(s): KILPATRICK, BRYAN T
• Application Number:
  US13/994,158
• Publication Number:
  US 20130343966A1
• Time in Patent Office: 2,315 Days
• Field of Search: 422504, 422502, 73 6771
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/024 Storing results with means integrated into the container
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

A microfluidic device for isolating particles of at least one given type of a sample; the device is designed to be connected to an apparatus through a plurality of electrical connectors and comprises a system of microfluidic channels and a flash memory, which contains information on the structure (arrangement and geometry of the various components) of the system of microfluidic channels, a map of non-functioning parts of the device, the maximum number of uses and the maximum time of use of the device; the memory has portions allocated for storing the number of times and the time of use of the device.

Claims

1. A microfluidic device for manipulating particles of at least one given type of a sample, the device (1) being designed to be connected to an apparatus;

the device comprising;

a connection unit (2) for connecting the device (1) itself to the apparatus, which is external to the device (1);

a microfluidic system (3), comprising;

at least one inlet (4;

4′

),

at least one outlet (5;

5′

),

at least one microfluidic chamber (6;

6′

) comprising a plurality of actuators within the chamber for selectively manipulating within the chamber the particles of the at least one given type with respect to other particles of the sample, and

a non-volatile rewritable memory disposed on the microfluidic device, wherein the memory contains information on the device, which information on the device comprises a map of positions of faulty actuators, and wherein the memory is adapted to be read by a control unit of the apparatus such that the control unit is configured to control the plurality of actuators of the device (1) to actuate so that the particles are moved in a path that does not pass a position corresponding to the positions of the faulty actuators.

2. The device according to claim 1, wherein the information on the device (1) is at least partially set in a non-rewritable area of the memory.

3. The device according to claim 1, wherein the actuators are electrodes;

and the identification of faulty actuators comprises an identification of the faulty electrodes.

4. The device according to claim 1, wherein the information on the device (1) further comprises information on the structure (arrangement and geometry of the different components) of the microfluidic system (3).

5. The device according to claim 1, wherein the information on the device (1) further comprises the maximum number of uses that can be performed.

6. The device according to claim 1, wherein the memory is designed to store the number of times that the device (1) is used;

in particular, a portion of the memory is allocated for storage of the number of times that the device (1) is used.

7. The device according to claim 1, wherein the information on the device (1) further comprises the maximum time of use of the device (1).

8. The device according to claim 1, wherein the memory is designed to store the time during which the device (1) is used;

in particular, a portion of the memory is allocated for storage of the time during which the device (1) is used.

9. The device according to claim 1, wherein the connection unit (2) comprises electrical connectors (2a) at least partially exposed towards the outside of the device (1).

10. The device according to claim 1, wherein the memory is accessible by means of a password.

11. The device according to claim 1, wherein the memory is encrypted.

12. The device according to claim 1, and comprising:

a silicon substructure (8), in the area of which the chamber (6;

6′

) is located;

an element (9) mainly made of plastic material, in which said inlet (4;

4′

), said outlet (5;

5′

), and at least one channel (7) of the microfluidic system (3) are obtained; and

a physical medium (10) for said memory, said physical medium (10) being set in an area corresponding to the element (9) mainly made of plastic material in a position different from the silicon substructure (8).

14. The device of claim 1, wherein the mapping of faulty actuators is read-only information written on the memory during testing of the microfluidic device prior to first use of the device.

13. A system, comprising:

an apparatus comprising a control unit; and

a microfluidic device comprising;

a connection unit for connecting the device to the apparatus,

a microfluidic system comprising;

at least one inlet,

at least one outlet,

at least one microfluidic chamber comprising a plurality of actuators for selectively moving particles of the given type within the at least one microfluidic chamber, and

a non-volatile rewritable memory disposed on the microfluidic device and containing information on the device, which information on the device comprises an identification of faulty actuators of the plurality of actuators and their respective locations;

wherein;

said microfluidic chamber is designed to move selectively the particles of the at least one given type with respect to other particles of the sample,

the apparatus is external to the microfluidic device,

the control unit of the apparatus controls the actuation of the plurality of actuators,

the control unit is configured to read the memory of the microfluidic device and control the actuation of the plurality of actuators so that the particles are moved in a path that does not pass a position where the faulty actuators are located.

15. The system of claim 13, wherein the identification of faulty actuators and their respective locations is read-only information written on the memory during testing of the microfluidic device prior to first use of the device.

16. The system of claim 13, wherein the actuators are electrodes.