Apparatus, method and system for generating optical radiation from biological gain media

• US 9,951,269 B2
• Filed: 04/10/2015
• Issued: 04/24/2018
• Est. Priority Date: 05/03/2010
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Yun, Seok-Hyun, Gather, Malte C.
• Primary Examiner(s): GOLUB-MILLER, MARCIA A
• Application Number:
  US14/683,985
• Publication Number:
  US 20150210920A1
• Time in Patent Office: 1,110 Days
• CPC Class Codes: C09K 11/06 containing organic luminescent materials
• CPC Class Codes: C09K 2211/10 Non-macromolecular compounds
• CPC Class Codes: C09K 2211/14 Macromolecular compounds
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/7746 the waveguide coupled to a cavity resonator
• CPC Class Codes: G02B 6/138 by using polymerisation
• CPC Class Codes: G02B 6/29341 Loop resonators operating in a whispering gallery mode evanescently coupled to a light guide, e.g. sphere or disk or cylinder evanescent coupling for sensing fluorescence G01N21/648
• CPC Class Codes: H01S 3/08022 Longitudinal modes mode suppression using a plurality of resonators H01S3/082
• CPC Class Codes: H01S 3/08059 Constructional details of the reflector, e.g. shape mirrors in general G02B5/08; mountings for mirrors G02B7/18
• CPC Class Codes: H01S 3/083 Ring lasers fibre ring lasers H01S3/06791
• CPC Class Codes: H01S 3/094034 the pumped medium being a dye
• CPC Class Codes: H01S 3/094076 Pulsed or modulated pumping H01S3/1024 takes precedence
• CPC Class Codes: H01S 3/168 using an organic dye dispersed in a solid matrix
• CPC Class Codes: H01S 3/20 Liquids
• CPC Class Codes: H01S 3/213 including an organic dye

Abstract

In one exemplary embodiment, an apparatus can be provided which includes at least one biological medium that causes gain. According to another exemplary embodiment, an arrangement can be provided which is configured to be provided in an anatomical structure. This exemplary arrangement can include at least one emitter having a cross-sectional area of at most 10 microns within the anatomical structure, and which is configured to generate at least one laser radiation. In a further exemplary embodiment, an apparatus can be provided which can include at least one medium which is configured to cause gain; and at least one optical biological resonator which is configured to provide an optical feedback to the medium. In still another exemplary embodiment, a process can be whereas, a solution of an optical medium can be applied to a substrate. Further, it is possible to generate a wave guide having a shape that is defined by (i) at least one property of the solution of the optical medium, or (ii) drying properties thereof.

Claims

1. An apparatus comprising at least one engineered or modified biological gain medium including at least one biologically-produced molecule that causes gain;

wherein the at least one biological medium includes at least two different fluorescent protein molecules,

wherein the at least two different fluorescent protein molecules are configured or structured to support a fluorescent resonant energy transfer from a first fluorescent protein molecule to a second fluorescent protein molecule, and

wherein the gain is provided by a stimulated emission in the at least one biological medium.

2. The apparatus according to claim 1, wherein the at least one biological medium includes biological molecules in at least one of a solution or a solid state within an anatomical structure.

3. The apparatus according to claim 1, wherein the at least two different fluorescent proteins are situated within at least one living cell.

4. The apparatus according to claim 1, further comprising at least one arrangement which is configured to pump the at least one biological medium to cause the gain.

5. The apparatus according to claim 4, wherein the at least one arrangement includes at least one of a bioluminescent source or a chemiluminescent source of an optical radiation.

6. The apparatus according to claim 4, wherein the at least one biological medium is configured to generate at least one electromagnetic radiation with at least one spectral peak.

7. The apparatus according to claim 4, further comprising at least one optical resonator configured to provide an optical feedback to the at least one biological medium.

8. The apparatus according to claim 7, wherein the at least one optical resonator at least partially includes at least one of a linear or ring cavity, photonic crystals, a biological tissue, a random scattering medium, a micro-scale reflecting chamber, a nano-scale reflecting chamber, plasmonic nano-particles, or includes a biological structure that is at least partially periodic.

9. The apparatus according to claim 1, wherein the least one biological medium is further configured to receive at least one first electro-magnetic radiation, and transmit at least one second electro-magnetic radiation, and wherein the least one biological medium is configured to amplify a magnitude of at least one of energy, power or intensity of the at least one first electro-magnetic radiation to produce the at least one second electro-magnetic radiation.

10. The apparatus according to claim 9, wherein the at least one second electro- magnetic radiation is the at least one amplified first electro-magnetic radiation.

11. The apparatus according to claim 1, wherein the at least one biological medium is configured to generate at least one of (i) at least one amplified spontaneous emission, or (ii) at least one laser emission.

12. The apparatus according to claim 11, further comprising a particular arrangement which is configured detect the at least one laser emission, and generate information as a function of the at least one laser emission.

13. The apparatus according to claim 12, further comprising a further arrangement which is configured generate at least one image of at least one of (i) the at least one biological medium, or (ii) at least one sample associated with the at least one biological medium using the information.

14. An apparatus comprising:

a substrate wherein at least one engineered or modified biological gain medium including a plurality of biologically-produced molecules that cause gain is deposited;

at least one arrangement which is configured to pump the at least on biological gain medium to cause the gain; and

wherein the at least one biological gain medium is configured as ring resonator to provide optical feedback.

15. The apparatus according to claim 14, wherein the plurality of biologically-produced molecules includes fluorescent proteins.

16. The apparatus according to claim 15, wherein the fluorescent proteins are selected from the group consisting of GFP, eGFP, turboRFP, DsRed, tdTomato, YFP, CFP, and mixtures thereof.

17. The apparatus according to claim 14, further comprising one or more fluorescent polymers configured within the circular waveguide.

18. The apparatus according to claim 17, wherein the one or more fluorescent polymers is selected from the group consisting of poly(p-phenylene vinylene), poly-co-fluorene, rhodamine, fluorescein, coumarin, stilbene, umbelliferone, tetracene, malachite green, and mixtures thereof.

19. The apparatus according to claim 14, wherein the at least one biological medium includes biological molecules in at least one of a solution or a solid state.

20. The apparatus according to claim 14, further comprising an optical fiber or a waveguide configured in proximity of the ring resonator, wherein evanescent coupling procedures can be used to extract energy.

21. The apparatus according to claim 20, wherein the distance between the ring resonator and the optical fiber or waveguide is between 10 nm to 100 μ

m.