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Methods of using IL-21 for adoptive immunotherapy and identification of tumor antigens

  • US 9,951,310 B2
  • Filed: 08/25/2014
  • Issued: 04/24/2018
  • Est. Priority Date: 11/24/2004
  • Status: Active Grant
First Claim
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1. A method of preparing a T cell population comprising:

  • isolating peripheral blood mononuclear cells (PBMCs) from a biological sample;

    identifying PBMCs having a histocompatible phenotype to a subject having a tumor;

    selecting a CD8+ human T cell population from the identified PBMCs;

    depleting CD45RO+ cells from the selected CD8+ human T cell population;

    selecting CD62L+ cells from the CD45RO+ cells depleted CD8+ human T cell population to obtain a naï

    ve CD8+ human T cell population having a phenotype of CD8+CD45RO-CD45RA+CD62L+,co-culturing tumor material isolated from the subject with the naï

    ve CD8+ human T cell population in the presence of an IL-21 composition and antigen presenting cells (APCs); and

    expanding the cells in culture.

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