Biosensor comprising electrode for measuring hematocrit value

• US 9,952,197 B2
• Filed: 04/05/2016
• Issued: 04/24/2018
• Est. Priority Date: 04/06/2015
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Saeda, Megumi, Kaneda, Hisashi
• Primary Examiner(s): Berke-Schlessel, David W
• Application Number:
  US15/090,639
• Publication Number:
  US 20160290987A1
• Time in Patent Office: 749 Days
• Field of Search: None
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/028 Circuits therefor measuring impedance per se G01R27/02
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence

Abstract

Provided is a method for measuring the concentration of a substance in a blood sample, comprising: supplying the blood sample to a biosensor comprising a hematocrit electrode for measuring a hematocrit value on the surface of which electrode an electrically conductive polymer is covalently immobilized; and calculating the concentration of the substance from the measured value of a first current resulting from application of a first voltage; and correcting the concentration of the substance with the value of a second current resulting from application of a second voltage or the hematocrit value calculated from the value of the second current.

Claims

1. A biosensor comprising:

an enzyme electrode configured to measure a glucose concentration in a blood sample, wherein a surface of the enzyme electrode is in contact with a detection layer comprising an oxidoreductase enzyme, a first electrically conductive polymer and electrically conductive particles; and

a hematocrit electrode configured to measure a hematocrit value in the blood sample, wherein the hematocrit electrode comprises a metal layer or a carbon layer formed on an insulating base plate and wherein a second electrically conductive polymer is covalently immobilized on a surface of the metal layer or the carbon layer by a crosslinking agent.

2. The biosensor according to claim 1, wherein the first and second electrically conductive polymers are water-soluble electrically conductive polymers.

3. The biosensor according to claim 1, wherein the first and second electrically conductive polymers are a polyaniline.

4. The biosensor according to claim 1, wherein the crosslinking agent contains at least one functional group selected from the group consisting of an aldehyde group, a maleimide group, a carbodiimide group, an oxazoline group, an epoxy group and combinations thereof.

5. The biosensor according to claim 1, further comprising a counter electrode and a reference electrode.

6. The biosensor according to claim 1, wherein the detection layer of the enzyme electrode further comprises a crosslinking agent.

7. The biosensor according to claim 1, wherein the oxidoreductase is saccharide oxidoreductase.

8. A method for reducing deviation in the measured concentration of blood glucose as an index of a disease or health status, comprising:

contacting a blood sample containing glucose with the detection layer of the enzyme electrode of the biosensor according to claim 1;

reacting the glucose in the blood sample with the oxidoreductase present in the detection layer by applying a first voltage of 100 to 300 mV to the enzyme electrode;

measuring the value of a resulting first current which flows from the first voltage, and calculating the concentration of the glucose from the measured value of the first current;

applying a second voltage of 400 to 800 mV to the hematocrit electrode;

measuring the value of a resulting second current which flows from the second voltage and calculating a hematocrit value from the measured value of the second current; and

correcting the measured glucose concentration to account for the hematocrit value.

9. The method according to claim 8, wherein the second voltage is 550 to 650 mV.

10. A measuring apparatus comprising:

the biosensor according to claim 1;

a control section configured to control the application of voltage to the enzyme electrode and the hematocrit electrode of the biosensor;

a detection section configured to measure the value of a first current which flows when a voltage is applied to the enzyme electrode, and the value of a second current which flows when a voltage is applied to the hematocrit electrode;

an arithmetic section configured to calculate the concentration of blood glucose from the value of the first current, and to correct the concentration of the blood glucose with the value of the second current or the hematocrit value calculated from the value of the second current; and

an output section configured to output the corrected concentration of the blood glucose to be measured.