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Devices and method for enrichment and alteration of cells and other particles

  • US 9,956,562 B2
  • Filed: 11/02/2015
  • Issued: 05/01/2018
  • Est. Priority Date: 04/05/2005
  • Status: Active Grant
First Claim
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1. A method of producing a sample enriched in particles of a specific type from a liquid sample, the method comprisingobtaining a liquid sample comprising particles of the specific type;

  • increasing a hydrodynamic size of particles of the specific type in the liquid sample by adding to the liquid sample a reagent that swells the particles of the specific type; and

    flowing the liquid sample through a channel under laminar flow conditions, wherein the channel comprises an inlet, an array of obstacles that form a network of gaps, and first and second outlets, wherein any particles in the liquid sample passing into the inlet and through the network of gaps are divided unequally into a major flux and a minor flux so that particles having a hydrodynamic size above a critical size are displaced laterally in the network of gaps to the first outlet and particles having a hydrodynamic size below the critical size are not displaced laterally in the network of gaps and flow to the second outlet, wherein the specific type of particles having an altered hydrodynamic size are directed selectively to either the first outlet or the second outlet, thereby producing a sample enriched in particles of the specific type.

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