Monoazo dyes with cyclic amine as fluorescence quenchers

• US 9,957,393 B2
• Filed: 03/30/2015
• Issued: 05/01/2018
• Est. Priority Date: 03/30/2015
• Status: Active Grant

• Current Assignee: Enzo Biochem Incorporated
• Original Assignee: Enzo Biochem Incorporated
• Inventors: Li, Zaiguo, Pande, Praveen, Raju, Natarajan
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US14/672,944
• Publication Number:
  US 20160289779A1
• Time in Patent Office: 1,128 Days
• Field of Search: 534798, 536 266
• CPC Class Codes: C09B 29/3643 from quinolines or hydrogenated quinolines
• CPC Class Codes: C09B 56/005 Azo-nitro dyes
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6846 Common amplification features
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 1/708 for papilloma
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels

Abstract

The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.

Claims

1. A compound selected from the group consisting of

2. The compound of claim 1 having the structure

3. The compound of claim 1 having the structure