Cell carrier, associated methods for making cell carrier and culturing cells using the same

• US 9,957,478 B2
• Filed: 11/07/2016
• Issued: 05/01/2018
• Est. Priority Date: 12/16/2010
• Status: Active Grant

• Current Assignee: Global Life Sciences Solutions USA LLC (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Davis, Brian Michael, Loghin, Evelina Roxana, Conway, Kenneth Roger, Burns, Andrew Arthur Paul, Gascoyne, David Gilles
• Primary Examiner(s): GALISTEO GONZALE, ANTONIO
• Application Number:
  US15/345,418
• Publication Number:
  US 20170051248A1
• Time in Patent Office: 540 Days
• Field of Search: None
• CPC Class Codes: B29C 37/0025 Applying surface layers, e.g. coatings, decorative layers, printed layers, to articles during shaping, e.g. in-mould printing moulding on preformed layers as inserts B29C70/68; applying fluent material to surfaces in general B05
• CPC Class Codes: B29C 59/02 by mechanical means, e.g. pressing B29C59/007 takes precedence; embossing expanded porous articles B29C44/5627
• CPC Class Codes: B29C 71/0081 using an electric field, e.g. for electrostatic charging electrostatic pinning of extruded material B29C48/9165; fixing linings by electrostatic charges B29C63/0043
• CPC Class Codes: B29K 2025/06 PS, i.e. polystyrene
• CPC Class Codes: B29K 2105/256 Sheets, plates, blanks or films
• CPC Class Codes: B29K 2883/00 Use of polymers having silicon, with or without sulfur, nitrogen, oxygen, or carbon only, in the main chain, as mould material
• CPC Class Codes: B29L 2031/712 Containers; Packaging elements or accessories, Packages closures therefor B29L2031/56; ink or toner cartridges B29L2031/7678; squeeze tubes B29L2023/20; suitcases B29L2031/7418
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12N 2501/999 Small molecules not provided for elsewhere
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 2537/00 Supports and/or coatings for cell culture characterised by physical or chemical treatment
• CPC Class Codes: C12N 2539/10 Coating allowing for selective detachment of cells, e.g. thermoreactive coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: Y10T 156/1039 Surface deformation only of sandwich or lamina [e.g., embossed panels]

Abstract

A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.

Claims

1. A method of making carriers for expanding pluripotent stem cells, comprising:

a) providing one or more flat polymer films;

b) forming on the one or more flat polymer films, one or more structured indentations;

c) cutting the one or more polymer films formed in step b) into a plurality of portions whereby the portions form carriers suitable for expanding pluripotent stem cells; and

d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof;

wherein step a) comprises providing two flat polymer films, and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated polymer film comprise a plurality of the structured indentations on opposing sides.

2. The method of claim 1, wherein the surface treatment comprises gas plasma treatment.

3. A method of making carriers for expanding pluripotent stem cells comprising:

a) providing one or more flat polymer films;

b) forming on the one or more flat polymer films, one or more structured indentations;

c) cutting the one or more polymer films formed in step b) into a plurality of portions to form carriers;

d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof;

e) disposing a biomolecular coating on the carriers formed by step d);

wherein step a) comprises providing two flat polymer films and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated film comprise a plurality of the structured indentations on opposing sides.

4. The method of claim 3, wherein the biomolecular coating comprises proteins, peptides or combinations thereof.

5. The method of claim 3, wherein the biomolecular coating comprises collagen, vitronectin, fibronectin, laminin or combinations thereof.

6. The method of claim 3, wherein the surface treatment comprises gas plasma treatment.

7. A method of making carriers for expanding pluripotent stem cells, comprising:

a) providing one or more flat polymer films;

b) forming on the one or more flat polymer films, one or more structured indentations;

c) cutting the one or more polymer films formed in step b) into a plurality of portions to form carriers;

d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof;

wherein a biomolecular coating is disposed on the carriers prior to imparting the surface treatment to the carriers; and

wherein step a) comprises providing two flat polymer films and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated film comprise a plurality of the structured indentations on opposing sides.

8. The method of claim 7, wherein the biomolecular coating comprises proteins, peptides or combinations thereof.

9. The method of claim 7, wherein the biomolecular coating comprises collagen, vitronectin, fibronectin, laminin or combinations thereof.

10. The method of claim 7, wherein the surface treatment comprises gas plasma treatment.