Cell carrier, associated methods for making cell carrier and culturing cells using the same
First Claim
1. A method of making carriers for expanding pluripotent stem cells, comprising:
- a) providing one or more flat polymer films;
b) forming on the one or more flat polymer films, one or more structured indentations;
c) cutting the one or more polymer films formed in step b) into a plurality of portions whereby the portions form carriers suitable for expanding pluripotent stem cells; and
d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof;
wherein step a) comprises providing two flat polymer films, and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated polymer film comprise a plurality of the structured indentations on opposing sides.
2 Assignments
0 Petitions
Accused Products
Abstract
A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
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Citations
10 Claims
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1. A method of making carriers for expanding pluripotent stem cells, comprising:
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a) providing one or more flat polymer films; b) forming on the one or more flat polymer films, one or more structured indentations; c) cutting the one or more polymer films formed in step b) into a plurality of portions whereby the portions form carriers suitable for expanding pluripotent stem cells; and d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof; wherein step a) comprises providing two flat polymer films, and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated polymer film comprise a plurality of the structured indentations on opposing sides. - View Dependent Claims (2)
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3. A method of making carriers for expanding pluripotent stem cells comprising:
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a) providing one or more flat polymer films; b) forming on the one or more flat polymer films, one or more structured indentations; c) cutting the one or more polymer films formed in step b) into a plurality of portions to form carriers; d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof; e) disposing a biomolecular coating on the carriers formed by step d); wherein step a) comprises providing two flat polymer films and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated film comprise a plurality of the structured indentations on opposing sides. - View Dependent Claims (4, 5, 6)
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7. A method of making carriers for expanding pluripotent stem cells, comprising:
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a) providing one or more flat polymer films; b) forming on the one or more flat polymer films, one or more structured indentations; c) cutting the one or more polymer films formed in step b) into a plurality of portions to form carriers; d) imparting a surface treatment to the carriers comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization, coating, or combinations thereof; wherein a biomolecular coating is disposed on the carriers prior to imparting the surface treatment to the carriers; and wherein step a) comprises providing two flat polymer films and step b) comprises forming a plurality of structured indentations on at least one surface of each of the two films, and laminating the two polymer films together to form a laminated film so that at least two outwardly facing surfaces of the laminated film comprise a plurality of the structured indentations on opposing sides. - View Dependent Claims (8, 9, 10)
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Specification