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Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples

  • US 9,957,557 B2
  • Filed: 08/12/2013
  • Issued: 05/01/2018
  • Est. Priority Date: 08/13/2012
  • Status: Active Grant
First Claim
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1. A process for assessing the extent of degradation of human DNA in a sample, the process comprising the steps of:

  • providing a sample containing human DNA;

    quantitating an amount of a first retrotransposon interspersed element in the sample using a real-time polymerase chain reaction (PCR) technique, and quantitating an amount of a second retrotransposon interspersed element in the sample using a real-time polymerase chain reaction technique, the first retrotransposon interspersed element being an Alu element found in the human genome, the second retrotransposon interspersed element being an SVA element of the human retinitis pigmentosa (RP) gene;

    determining a ratio of the amount of the first retrotransposon interspersed element to the amount of the second retrotransposon interspersed element in the sample; and

    assessing the extent of degradation of human DNA in the sample based on said ratio.

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