Method for measuring hematocrit value of blood sample, method for measuring concentration of analyte in blood sample, sensor chip and sensor unit

• US 9,958,411 B2
• Filed: 12/09/2015
• Issued: 05/01/2018
• Est. Priority Date: 10/19/2006
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Fujiwara, Masaki, Ikeda, Shin, Nakaminami, Takahiro
• Primary Examiner(s): Dinh, Bach T
• Application Number:
  US14/964,128
• Publication Number:
  US 20160091453A1
• Time in Patent Office: 874 Days
• Field of Search: 20440301-40315, 2057775-778
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 27/413 Concentration cells using liquid electrolytes measuring currents or voltages in voltaic cells

Abstract

Provided is a sensor chip for electrochemically measuring a concentration of an analyte in a blood sample. In one embodiment of the sensor, the sensor chip includes a substrate, and a preliminary measurement analyzer and a hematocrit value analyzer disposed on the substrate. The preliminary measurement analyzer includes a preliminary working electrode and a preliminary counter electrode. The hematocrit value analyzer includes a working electrode and a counter electrode. An oxidant of a redox substance is disposed on the preliminary measurement analyzer and the counter electrode. A reductant of a redox substance is disposed on the working electrode.

Claims

1. A method of determining an amount of a component in blood using a biosensor,

the biosensor comprising:

a first electrode,

a second electrode,

an oxidant of a redox substance, and

a reductant of a redox substance,

wherein the reductant of a redox substance is disposed only on the first electrode, and

the oxidant of a redox substance is disposed only on the second electrode;

the method comprising;

applying a first voltage to the first electrode and the second electrode such that the first electrode acts as a working electrode and the second electrode acts as a counter electrode;

detecting a first current value generated by the application of the first voltage;

applying a second voltage to the second electrode such that the second electrode acts as a working electrode;

detecting a second current value generated by the application of the second voltage; and

calculating an amount of the component using the first current value and the second current value.

2. The method of claim 1, wherein the first voltage is applied for a time of 0.01 to 10 seconds.

3. The method of claim 1, wherein the second voltage is applied for a time of 0.1 to 10 seconds.

4. The method of claim 1, wherein the amount of the component is calculated taking into account the amount of hematocrit present in the blood.

5. The method of claim 1, wherein the first and second electrode comprise gold or palladium or carbon.

6. The method of claim 1, wherein a redox enzyme is disposed on the second electrode.