Composition having tissue-repairing activity, and use therefor

• US 9,962,428 B2
• Filed: 12/24/2013
• Issued: 05/08/2018
• Est. Priority Date: 12/21/2012
• Status: Active Grant

• Current Assignee: Tokushima University
• Original Assignee: Nagoya University
• Inventors: Yamamoto, Akihito, Ueda, Minoru, Matsubara, Kohki, Suzumura, Akio, Furukawa, Koichi, Matsushita, Yoshihiro, Wakayama, Hirotaka, Takahashi, Nobunori, Tsunekawa, Shin, Izumoto, Takako
• Primary Examiner(s): Steele, Amber D
• Application Number:
  US14/654,624
• Publication Number:
  US 20150352187A1
• Time in Patent Office: 1,596 Days
• Field of Search: None
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/737 Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate A61K31/727 takes precedence
• CPC Class Codes: A61K 38/178 Lectin superfamily, e.g. selectins
• CPC Class Codes: A61K 38/195 Chemokines, e.g. RANTES
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 21/02 Muscle relaxants, e.g. for tetanus or cramps
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 27/04 Artificial tears; Irrigation solutions
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 : for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C12N 2501/21 : Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
• CPC Class Codes: C12N 2501/59 : Lectins
• CPC Class Codes: C12N 2501/90 : Polysaccharides
• CPC Class Codes: C12N 5/0645 : Macrophages, e.g. Kuepfer cells in the liver; Monocytes

Abstract

A composition having tissue repair activity, which is capable of promoting reactions associated with tissue repair, contains at least one selected from the group consisting of a first component that is a protein having a monocyte chemotactic protein-1 (MCP-1) activity, a second component that is a protein having the extracellular domain activity of sialic acid-binding immunoglobulin-type lectin-9 (Siglec-9), and a third component that is at least one of chondroitin sulfate and chondroitin sulfate proteoglycan.

Claims

1. A method for treating hepatitis comprising administering, to a subject in need thereof, a composition comprising:

a component comprising an extracellular domain activity of sialic acid-binding immunoglobulin-type lectin-9 (Siglec-9), the component not having a cytoplasmic domain of Siglec-9; and

a component comprising a monocyte chemotactic protein-1 (MCP-1) activity.

2. The method according to claim 1, wherein the component comprising an extracellular domain activity of Siglec-9 has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

4.

3. The method according to claim 1, wherein the component comprising the MCP-1 activity has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

2.

4. The method according to claim 1, wherein the composition further comprises at least one bioabsorbable material, carrier, excipient, disintegrator, buffer, emulsifier, suspension agent, soothing agent, stabilizer, preservative, antiseptic agent, physiological saline, or pharmaceutically acceptable salt.

5. The method according to claim 1, wherein the composition is formulated into a dosage form selected from a pill, a powder, a grain, a granule, a capsule, an injectable composition, a suppository, an infusion, drops, a spray, a lotion, a cream, a patch, or a topical preparation.

6. A method for treating severe hepatitis comprising administering, to a subject in need thereof, a composition comprising:

a component comprising an extracellular domain activity of sialic acid-binding immunoglobulin-type lectin-9 (Siglec-9), the component not having a cytoplasmic domain of Siglec-9; and

a component comprising a monocyte chemotactic protein-1 (MCP-1) activity.

7. The method according to claim 6, wherein the component comprising an extracellular domain activity of Siglec-9 has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

4.

8. The method according to claim 6, wherein the component comprising the MCP-1 activity has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

2.

9. The method according to claim 6, wherein the composition further comprises at least one bioabsorbable material, carrier, excipient, disintegrator, buffer, emulsifier, suspension agent, soothing agent, stabilizer, preservative, antiseptic agent, physiological saline, or pharmaceutically acceptable salt.

10. The method according to claim 6, wherein the composition is formulated into a dosage form selected from a pill, a powder, a grain, a granule, a capsule, an injectable composition, a suppository, an infusion, drops, a spray, a lotion, a cream, a patch, or a topical preparation.

11. A method of treating a spinal cord injury comprising administering, to a subject in need thereof, a composition comprising:

a component comprising an extracellular domain activity of sialic acid-binding immunoglobulin-type lectin-9 (Siglec-9), the component not having a cytoplasmic domain of Siglec-9;

a component comprising a monocyte chemotactic protein-1 (MCP-1) activity; and

chondroitin sulfate proteoglycan.

12. The method according to claim 11, wherein the component comprising an extracellular domain activity of Siglec-9 has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

4.

13. The method according to claim 11, wherein the component comprising the MCP-1 activity has an amino acid sequence having 90% or more identity with the amino acid sequence represented by SEQ ID NO:

2.

14. The method according to claim 11, wherein the composition further comprises at least one bioabsorbable material, carrier, excipient, disintegrator, buffer, emulsifier, suspension agent, soothing agent, stabilizer, preservative, antiseptic agent, physiological saline, or pharmaceutically acceptable salt.

15. The method according to claim 11, wherein the composition is formulated into a dosage form selected from a pill, a powder, a grain, a granule, a capsule, an injectable composition, a suppository, an infusion, drops, a spray, a lotion, a cream, a patch, or a topical preparation.