Cartridges, kits, and methods for amplification and detection of analytes
First Claim
1. A method of amplification for determining at least one of a presence, absence, or quantity of a target nucleic acid in a sample, the method comprising:
- introducing the sample comprising the target nucleic acid into fluid held by a reservoir of a microfluidic cartridge and introducing a reagent ball into the fluid held by the reservoir, the reagent ball comprising reagents for amplification of the target nucleic acid by an amplification reaction;
amplifying the target nucleic acid with an effective amount of the reagents for amplification in the reservoir to generate amplicons comprising signaling agents;
introducing the fluid comprising the amplicons into an analysis channel from the reservoir such that the amplicons indicative of at least one of a presence, absence, or quantity of the target nucleic acid in the sample localize over a sensor at least partially disposed in the analysis channel;
reacting the signaling agents with a substrate; and
sensing signals indicative of at least one of the presence, absence, or quantity of the target nucleic acid in the sample based on reactions between the substrate and the signaling agents of the amplicons localized over the sensor.
6 Assignments
0 Petitions
Accused Products
Abstract
Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
-
Citations
14 Claims
-
1. A method of amplification for determining at least one of a presence, absence, or quantity of a target nucleic acid in a sample, the method comprising:
-
introducing the sample comprising the target nucleic acid into fluid held by a reservoir of a microfluidic cartridge and introducing a reagent ball into the fluid held by the reservoir, the reagent ball comprising reagents for amplification of the target nucleic acid by an amplification reaction; amplifying the target nucleic acid with an effective amount of the reagents for amplification in the reservoir to generate amplicons comprising signaling agents; introducing the fluid comprising the amplicons into an analysis channel from the reservoir such that the amplicons indicative of at least one of a presence, absence, or quantity of the target nucleic acid in the sample localize over a sensor at least partially disposed in the analysis channel; reacting the signaling agents with a substrate; and sensing signals indicative of at least one of the presence, absence, or quantity of the target nucleic acid in the sample based on reactions between the substrate and the signaling agents of the amplicons localized over the sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
Specification