Sugar mixtures and methods for production and use thereof

• US 9,963,673 B2
• Filed: 06/23/2016
• Issued: 05/08/2018
• Est. Priority Date: 06/26/2010
• Status: Active Grant

• Current Assignee: Virdia Ltd
• Original Assignee: Virdia, Inc. (Stora Enso Oyj)
• Inventors: Eyal, Aharon Meir, Jansen, Robert P., Mali, Revital, Vitner, Asher
• Primary Examiner(s): Heincer, Liam J
• Application Number:
  US15/191,376
• Publication Number:
  US 20160376546A1
• Time in Patent Office: 684 Days
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/60 Sugars, e.g. mono-, di-, tri-, tetra-saccharides
• CPC Class Codes: C01B 7/0737 hydrogen chloride being extracted
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: C13K 13/00 Sugars not otherwise provided for in this class
• CPC Class Codes: C13K 7/00 Maltose
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≥0.06 to total saccharides; disaccharides in a ratio to total saccharides ≥0.05; pentose in a ratio to total saccharides ≥0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

Claims

1. A lignocellulosic hydrolysate comprising:

monosaccharides;

higher oligosaccharides comprising three or more sugar units;

a ratio of pentose to total saccharides of ≥

0.05 weight/weight;

at least one alpha-bonded di-glucose;

at least one beta-bonded di-glucose;

a ratio of mineral acid to total saccharides of ≤

0.03 weight/weight; and

a ratio of total saccharides to water of ≥

0.35 weight/weight,

wherein a ratio of the higher oligosaccharides to total saccharides is ≤

0.2 weight/weight.

2. The hydrolysate of claim 1, wherein a ratio of at least one of the alpha-bonded di-glucose and the beta-bonded di-glucose to total saccharides is ≥

0.01 weight/weight.

3. The hydrolysate of claim 1, wherein the at least one alpha-bonded di-glucose comprises maltose, isomaltose or trehalose.

4. The hydrolysate of claim 1, wherein the at least one beta-bonded di-glucose comprises gentiobiose, sophorose or cellobiose.

5. The hydrolysate of claim 1, wherein the monosaccharides comprise glucose, galactose, mannose, xylose and arabinose.

6. The hydrolysate of claim 1, further comprising hexanol or 2-ethyl-1-hexanol.

7. The hydrolysate of claim 1, further comprising a microorganism.

8. The hydrolysate of claim 7, wherein the microorganism is a genetically modified microorganism.

9. The hydrolysate of claim 1, further comprising a catalyst, wherein the catalyst is not an acid or an enzyme.

10. The hydrolysate of claim 1, wherein the hydrolysate is de-salted.

11. A method comprising:

providing the lignocellulosic hydrolysate of claim 1; and

converting the saccharides in the hydrolysate to a conversion product.

12. The method of claim 11, wherein the converting comprises fermentation.

13. The method of claim 11, wherein the converting comprises catalytic conversion.

14. The method of claim 11, wherein the conversion product is selected from the group consisting of alcohols, carboxylic acids, amino acids, monomers for the polymer industry and proteins.

15. The method of claim 11, wherein the conversion product is selected from the group consisting of ethanol, butanol, isobutanol, a fatty acid, a fatty acid ester, a fatty alcohol and biodiesel.

16. The method of claim 11, further comprising converting the conversion product to a consumer product or an ingredient of a consumer product.

17. The method of claim 11, wherein the conversion product is characterized by a ratio of carbon-14 to carbon-12 of about 2.0×

10<sup>−

13</sup> or greater.

18. A fermentation media comprising:

monosaccharide hydrolysates;

higher oligosaccharide hydrolysates comprising three or more sugar units;

a ratio of pentose to total saccharides of ≥

0.05 weight/weight;

at least one alpha-bonded di-glucose;

at least one beta-bonded di-glucose;

a ratio of mineral acid to total saccharides of ≤

0.03 weight/weight; and

a microorganism.

19. A lignocellulosic hydrolysate comprising:

monosaccharides;

higher oligosaccharides comprising three or more sugar units;

a ratio of pentose to total saccharides of ≥

0.05 weight/weight;

at least one alpha-bonded di-glucose;

at least one beta-bonded di-glucose; and

a ratio of mineral acid to total saccharides of ≤

0.03 weight/weight,

wherein a ratio of the higher oligosaccharides to total saccharides is ≤

0.2 weight/weight, and

wherein the lignocellulosic hydrolysate is capable of use in a catalytic conversion process for converting monosaccharides into a conversion product.