Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
First Claim
1. A method of creating a chemical array, the method comprising:
- a) providing a platen having a plurality of through-holes and two opposing surfaces;
b) applying a mask to one or both surfaces of the platen to block at least some of the through-holes, while leaving other through-holes open;
c) exposing a surface of the platen to a reagent so that the reagent enters at least one of the open through-holes; and
d) repeating steps b) and c) with at least one different mask and at least one different reagent to create a chemical array;
loading at least some of the through-holes with a liquid sample comprising a biological compound;
performing a polymerase chain reaction on at least some of the biological compounds.
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Accused Products
Abstract
The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.
42 Citations
10 Claims
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1. A method of creating a chemical array, the method comprising:
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a) providing a platen having a plurality of through-holes and two opposing surfaces; b) applying a mask to one or both surfaces of the platen to block at least some of the through-holes, while leaving other through-holes open; c) exposing a surface of the platen to a reagent so that the reagent enters at least one of the open through-holes; and d) repeating steps b) and c) with at least one different mask and at least one different reagent to create a chemical array; loading at least some of the through-holes with a liquid sample comprising a biological compound; performing a polymerase chain reaction on at least some of the biological compounds. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A device for filling an array of wells in a platen, the device comprising:
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a platen comprising an array of wells, the plurality of wells configured to receive a reagent each; a holder adapted to accept the platen; a nozzle comprising an aperture configured to inject a sample comprising a biological compound into at least some of wells; and a valve that controls a flow of a sample through the nozzle, wherein the holder and nozzle can move with respect to each other. - View Dependent Claims (10)
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Specification