Devices for cell assays
First Claim
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1. A cell assay device comprising:
- a multiwell plate comprising a plurality of wells having one or more cell assay zones on the bottom surface of said wells in said multiwell plate, said assay zones comprising one or more spatially distinct cell seeding zones comprising a surface to which cells can adhere positioned adjacent to one or more spatially distinct cell exclusion zones comprising a blocking material deposited on said bottom surface of said wells to block adherence of cells to the surface of said cell exclusion zone, wherein said blocking material is selected from the group consisting of poly n-butyl hemiester of [poly(maleic anhydride-alt-2-methoxyethyl-vinyl ether), poly N-isopropylacrylamide, poly(lactic acid), poly[(lactic acid-co-(glycolic acid)], poly(ethylene oxide), poly(propylene oxide), N-isopropylacrylamide copolymers, cellulose acetate butyrate polymers, perfluorocarbon terminated polymers, poly(N,N-diethylacrylamide), N-isopropylacrylamide, diethyleneglycol methacrylate (poly(NiPAAm-co-DEGMA) copolymers, alginate, starch glycogen, chitin, xanthan gum, dextran, gellan gum, glucomannan, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose, carageenan, inulin, agarose, polyvinyl alcohol, pullulan, and nucleic acids, wherein said blocking material dissolves upon exposure to an aqueous cell culture medium to expose a surface to which cells can adhere.
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Abstract
The present invention relates to the field of molecular diagnostics. In particular, the present invention provided improved substrates and methods of using liquid crystals and other biophotonically based assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal or other biophotonically based assay formats.
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8 Claims
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1. A cell assay device comprising:
a multiwell plate comprising a plurality of wells having one or more cell assay zones on the bottom surface of said wells in said multiwell plate, said assay zones comprising one or more spatially distinct cell seeding zones comprising a surface to which cells can adhere positioned adjacent to one or more spatially distinct cell exclusion zones comprising a blocking material deposited on said bottom surface of said wells to block adherence of cells to the surface of said cell exclusion zone, wherein said blocking material is selected from the group consisting of poly n-butyl hemiester of [poly(maleic anhydride-alt-2-methoxyethyl-vinyl ether), poly N-isopropylacrylamide, poly(lactic acid), poly[(lactic acid-co-(glycolic acid)], poly(ethylene oxide), poly(propylene oxide), N-isopropylacrylamide copolymers, cellulose acetate butyrate polymers, perfluorocarbon terminated polymers, poly(N,N-diethylacrylamide), N-isopropylacrylamide, diethyleneglycol methacrylate (poly(NiPAAm-co-DEGMA) copolymers, alginate, starch glycogen, chitin, xanthan gum, dextran, gellan gum, glucomannan, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, carboxymethyl cellulose, carageenan, inulin, agarose, polyvinyl alcohol, pullulan, and nucleic acids, wherein said blocking material dissolves upon exposure to an aqueous cell culture medium to expose a surface to which cells can adhere. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
Specification