Differentiation of human embryonic stem cells
First Claim
1. A method for producing pancreatic endoderm cells comprising:
- a. differentiating human pluripotent stem cells into definitive endoderm cells; and
b. differentiating the definitive endoderm cells into pancreatic endoderm cells by treating the definitive endoderm cells with a netrin and at least one factor selected from the group consisting of retinoic acid, FGF-2, FGF-4, FGF-7, FGF-10, a sonic hedgehog inhibitor, and a factor capable of inhibiting BMP.
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Abstract
The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells.
159 Citations
31 Claims
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1. A method for producing pancreatic endoderm cells comprising:
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a. differentiating human pluripotent stem cells into definitive endoderm cells; and b. differentiating the definitive endoderm cells into pancreatic endoderm cells by treating the definitive endoderm cells with a netrin and at least one factor selected from the group consisting of retinoic acid, FGF-2, FGF-4, FGF-7, FGF-10, a sonic hedgehog inhibitor, and a factor capable of inhibiting BMP. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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- 28. A method of differentiating human definitive endoderm cells into pancreatic endoderm cells comprising treating the definitive endoderm cells with FGF-7, cyclopamine, retinoic acid, noggin and a netrin.
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31. A method of differentiating human definitive endoderm cells into pancreatic endoderm cells by treating the definitive endoderm cells with a netrin and at least one factor selected from the group consisting of retinoic acid, FGF-2, FGF-4, FGF-7, FGF-10, a sonic hedgehog inhibitor, and a factor capable of inhibiting BMP.
Specification