×

Methods and products for transfection

  • US 9,969,983 B2
  • Filed: 12/15/2017
  • Issued: 05/15/2018
  • Est. Priority Date: 12/05/2011
  • Status: Active Grant
First Claim
Patent Images

1. A method for reprogramming a non-pluripotent cell comprising:

  • (a) providing a non-pluripotent cell, the non-pluripotent cell being derived from a biopsy of a human subject;

    (b) culturing the non-pluripotent cell; and

    (c) transfecting the non-pluripotent cell with a synthetic RNA molecule, wherein;

    the synthetic RNA molecule encodes one or more reprogramming factor(s) selected from the group consisting of Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, I-Myc protein, Tert protein, Nanog protein, and Lin28 protein,the transfecting results in the non-pluripotent cell expressing the one or more reprogramming factor(s) which reprograms the non-pluripotent cell; and

    step (c) is performed without using irradiated human neonatal fibroblast feeder cells and occurs in the presence of a medium containing ingredients that support reprogramming of the non-pluripotent cell.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×