Treatment of atonal homolog 1 (ATOH1) related diseases by inhibition of natural antisense transcript to ATOH1
First Claim
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1. A method of identifying at least one modified oligonucleotide of 10 to 30 nucleotides in length which specifically hybridizes to a natural antisense transcript of a ATOH1 polynucleotide and modulates the expression of said ATOH1 polynucleotide comprising:
- identifying at least one oligonucleotide comprising 10-30 consecutive nucleotides which is at least 90% complementary to a natural antisense polynucleotide of the ATOH1 polynucleotide;
measuring the thermal melting point of a hybrid of said antisense oligonucleotide and the natural antisense polynucleotide of said ATOH1 polynucleotide under stringent hybridization conditions;
measuring the ATOH1 polynucleotide modulatory activity of the antisense oligonucleotide in vitro and identifying at least one antisense oligonucleotide which modulates the expression of said ATOH1 polynucleotide in vitro.
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Abstract
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Atonal homolog 1 (ATOH1), in particular, by targeting natural antisense polynucleotides of Atonal homolog 1 (ATOH1). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of ATOH1.
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13 Claims
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1. A method of identifying at least one modified oligonucleotide of 10 to 30 nucleotides in length which specifically hybridizes to a natural antisense transcript of a ATOH1 polynucleotide and modulates the expression of said ATOH1 polynucleotide comprising:
- identifying at least one oligonucleotide comprising 10-30 consecutive nucleotides which is at least 90% complementary to a natural antisense polynucleotide of the ATOH1 polynucleotide;
measuring the thermal melting point of a hybrid of said antisense oligonucleotide and the natural antisense polynucleotide of said ATOH1 polynucleotide under stringent hybridization conditions;
measuring the ATOH1 polynucleotide modulatory activity of the antisense oligonucleotide in vitro and identifying at least one antisense oligonucleotide which modulates the expression of said ATOH1 polynucleotide in vitro. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- identifying at least one oligonucleotide comprising 10-30 consecutive nucleotides which is at least 90% complementary to a natural antisense polynucleotide of the ATOH1 polynucleotide;
Specification