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Treatment of atonal homolog 1 (ATOH1) related diseases by inhibition of natural antisense transcript to ATOH1

  • US 9,970,008 B2
  • Filed: 02/08/2017
  • Issued: 05/15/2018
  • Est. Priority Date: 05/26/2010
  • Status: Active Grant
First Claim
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1. A method of identifying at least one modified oligonucleotide of 10 to 30 nucleotides in length which specifically hybridizes to a natural antisense transcript of a ATOH1 polynucleotide and modulates the expression of said ATOH1 polynucleotide comprising:

  • identifying at least one oligonucleotide comprising 10-30 consecutive nucleotides which is at least 90% complementary to a natural antisense polynucleotide of the ATOH1 polynucleotide;

    measuring the thermal melting point of a hybrid of said antisense oligonucleotide and the natural antisense polynucleotide of said ATOH1 polynucleotide under stringent hybridization conditions;

    measuring the ATOH1 polynucleotide modulatory activity of the antisense oligonucleotide in vitro and identifying at least one antisense oligonucleotide which modulates the expression of said ATOH1 polynucleotide in vitro.

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