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Synthesis of long-chain polyunsaturated fatty acids by recombinant cell

  • US 9,970,033 B2
  • Filed: 07/07/2017
  • Issued: 05/15/2018
  • Est. Priority Date: 04/22/2004
  • Status: Active Grant
First Claim
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1. A Brassica plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell comprisinga polynucleotide which encodes a Δ

  • 4 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;

    a polynucleotide which encodes a Δ

    5 desaturase which has the amino acid sequence set forth as SEQ ID NO;

    18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;

    a polynucleotide which encodes a Δ

    6 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; and

    a polynucleotide which encodes a Δ

    5/Δ

    6 bifunctional elongase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell.

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