Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
First Claim
1. A Brassica plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell comprisinga polynucleotide which encodes a Δ
- 4 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
5 desaturase which has the amino acid sequence set forth as SEQ ID NO;
18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
6 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; and
a polynucleotide which encodes a Δ
5/Δ
6 bifunctional elongase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell.
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Abstract
The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.
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Citations
34 Claims
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1. A Brassica plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell comprising
a polynucleotide which encodes a Δ - 4 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
a polynucleotide which encodes a Δ
5 desaturase which has the amino acid sequence set forth as SEQ ID NO;
18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
6 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; anda polynucleotide which encodes a Δ
5/Δ
6 bifunctional elongase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell. - View Dependent Claims (2, 3, 4, 5, 24, 31, 32)
- 4 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;
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6. A Brassica plant cell comprising
a polynucleotide which encodes a Δ - 4 desaturase which has the amino acid sequence encoded by a Δ
4 desaturase gene from Pavlova lutheri, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
5 desaturase which has the amino acid sequence set forth as SEQ ID NO;
18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
6 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; anda polynucleotide which encodes a Δ
5/Δ
6 bifunctional elongase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 25, 26, 27, 33)
- 4 desaturase which has the amino acid sequence encoded by a Δ
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15. A Brassica plant cell comprising
a polynucleotide which encodes a Δ - 4 desaturase which has the amino acid sequence encoded by a Δ
4 desaturase gene from Pavlova lutheri, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
5 desaturase which has the amino acid sequence set forth as SEQ ID NO;
18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ
6 desaturase operably linked to a promoter which directs expression of the polynucleotide in the plant cell; anda polynucleotide which encodes a Δ
6 elongase which has the amino acid sequence set forth as SEQ ID NO;
31, operably linked to a promoter which directs expression of the polynucleotide in the plant cell. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 28, 29, 30, 34)
- 4 desaturase which has the amino acid sequence encoded by a Δ
Specification