Bioagent detection oligonucleotides
First Claim
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1. A method of detecting bacterial DNA in the presence of contaminating human DNA comprising:
- a) contacting a sample comprising human genomic DNA, contaminants, and possibly bacterial DNA with an oligonucleotide wherein said oligonucleotide is 1 a sequence selected from SEQ ID NOs;
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b) allowing said oligonucleotide to hybridize to bacterial DNA, if bacterial DNA is present in said sample; and
c) detecting said bacterial DNA in the presence of contaminating human DNA, if bacterial DNA is present in said sample, based upon hybridization of said oligonucleotide to said bacterial DNA.
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Abstract
The present invention compositions, methods and systems to identify, detect, and/or quantify bacterial DNA in the presence of contaminating non-bacterial DNA. In particular, the present invention provides oligonucleotides configured to detect a relatively small amount of bacterial DNA in the presence of an overwhelmingly large amount of contaminating human DNA.
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Citations
12 Claims
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1. A method of detecting bacterial DNA in the presence of contaminating human DNA comprising:
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a) contacting a sample comprising human genomic DNA, contaminants, and possibly bacterial DNA with an oligonucleotide wherein said oligonucleotide is 1 a sequence selected from SEQ ID NOs;
1-18;b) allowing said oligonucleotide to hybridize to bacterial DNA, if bacterial DNA is present in said sample; and c) detecting said bacterial DNA in the presence of contaminating human DNA, if bacterial DNA is present in said sample, based upon hybridization of said oligonucleotide to said bacterial DNA. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification