Multipotent adult stem cells and methods for isolation

• US 9,974,811 B2
• Filed: 04/01/2009
• Issued: 05/22/2018
• Est. Priority Date: 08/05/1999
• Status: Expired due to Term

• Current Assignee: ABT Holding Company (Athersys, Inc.)
• Original Assignee: ABT Holding Company (Athersys, Inc.)
• Inventors: Furcht, Leo T., Verfaillie, Catherine M., Reyes, Morayma
• Primary Examiner(s): Berke-Schlessel, David W
• Application Number:
  US12/416,700
• Publication Number:
  US 20090233354A1
• Time in Patent Office: 3,338 Days
• Field of Search: None
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2039/5156 expressing foreign proteins
• CPC Class Codes: A61K 2239/31 characterized by the route of administration
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 39/461 characterised by the cell type used
• CPC Class Codes: A61K 39/46432 Nervous system antigens
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12Y 207/07049 RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase

Abstract

The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells.

Claims

1. A cell culture comprising isolated expanded human non-embryonic, non-germ cells that are obtained by culture of non-embryonic, non-germ tissue, the cells having undergone 40 cell doublings in culture, wherein the cells have a normal karyotype.

2. The cell culture of claim 1 wherein the non-embryonic, non-germ cells express one or more of oct4, telomerase, rex-1, rox-1, or sox-2.

3. The cell culture of claim 1 wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages.

4. The cell culture of claim 3 wherein the non-embryonic, non-germ cells express one or more of oct4, telomerase, rex-1, rox-1, or sox-2.

5. The cell culture of claim 3 wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages.

6. The cell culture of claim 5 wherein the non-embryonic, non-germ cells express one or more of oct4, telomerase, rex-1, rox-1, or sox-2.

7. A pharmaceutical composition comprising the isolated expanded non-embryonic, non-germ cells of claim 1 in a pharmaceutically acceptable carrier.

8. A method for making the pharmaceutical composition of claim 7 comprising admixing the isolated expanded non-embryonic, non-germ cells with a pharmaceutically acceptable carrier.

9. A method for making a cell culture composition, said method comprising introducing the isolated expanded human non-embryonic, non-germ cells of claim 1 into cell culture medium.

10. The cell culture of any of claim 1-6 wherein the isolated expanded human non-embryonic, non-germ cells are derived from bone marrow.

11. The pharmaceutical composition of claim 7 wherein the isolated expanded human non-embryonic, non-germ cells are derived from bone marrow.

12. The method of claim 8 wherein the isolated expanded human non-embryonic, non-germ cells are derived from bone marrow.

13. The method of claim 9 wherein the isolated expanded human non-embryonic, non-germ cells are derived from bone marrow.