Nucleic acids encoding modified FGF-21 polypeptides comprising non-naturally occurring amino acids
First Claim
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1. An isolated nucleic acid that encodes a biologically active modified FGF-21 polypeptide comprising a non-naturally encoded amino acid, wherein:
- (a) the modified FGF-21 polypeptide comprises a sequence at least 95% identical to SEQ ID NO;
1 fused to an N-terminal methionine, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5 fused to an N-terminal methionine, SEQ ID NO;
6, or SEQ ID NO;
7;
(b) said non-naturally encoded amino acid has the structure;
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Abstract
Modified FGF-21 polypeptides and uses thereof are provided.
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Citations
20 Claims
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1. An isolated nucleic acid that encodes a biologically active modified FGF-21 polypeptide comprising a non-naturally encoded amino acid, wherein:
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(a) the modified FGF-21 polypeptide comprises a sequence at least 95% identical to SEQ ID NO;
1 fused to an N-terminal methionine, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5 fused to an N-terminal methionine, SEQ ID NO;
6, or SEQ ID NO;
7;(b) said non-naturally encoded amino acid has the structure; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. An isolated nucleic acid that encodes a biologically active modified FGF-21 polypeptide comprising a non-naturally encoded amino acid, wherein:
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(a) the modified FGF-21 polypeptide comprises the sequence of SEQ ID NO;
1 fused to an N-terminal methionine having said non-naturally encoded amino acid substituted at position 108 of SEQ ID NO;
1;(b) said non-naturally encoded amino acid has the structure; - View Dependent Claims (10, 11, 12)
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13. An isolated nucleic acid that encodes a modified FGF-21 polypeptide, wherein:
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(a) the modified FGF-21 polypeptide comprises SEQ ID NO;
1 fused to an N-terminal methionine, except that residue 108 of SEQ ID NO;
1 is substituted by a non-naturally encoded amino acid comprising para-acetyl-L-phenylalanine; and(b) said non-naturally encoded amino acid is encoded by a selector codon. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification