Nucleic acid sequencing technique using a pH-sensing agent
First Claim
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1. An apparatus for determining a sequence of nucleotides in nucleic acids, the apparatus comprising:
- a light source for emitting excitation light;
a device having a surface for receiving an object, the object directly contacting the surface or in close proximity thereto when received thereby, the object having a pH-sensing agent bound thereto or in close proximity to the object, the device including a detector operative for detecting light emitted or absorbed by the pH-sensing agent in response to the excitation light emitted by the light source;
a first high-pass optical interference filter or a first dichroic mirror disposed over the detector, the first optical interference filter or the first dichroic mirror operative for allowing the light emitted by the pH-sensing agent to pass therethrough; and
a second optical low-pass interference filter or a second dichroic mirror disposed between the light source and the surface of the device, the second optical interference filter or the second dichroic mirror operative for allowing the certain wavelengths of the excitation light emitted by the light source to pass therethrough and for reflecting light emitted by the pH-sensing agent.
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Abstract
The invention relates to a composition, method and apparatus for determining the sequence of a nucleic acid strand utilizing a pH-sensing agent.
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Citations
21 Claims
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1. An apparatus for determining a sequence of nucleotides in nucleic acids, the apparatus comprising:
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a light source for emitting excitation light; a device having a surface for receiving an object, the object directly contacting the surface or in close proximity thereto when received thereby, the object having a pH-sensing agent bound thereto or in close proximity to the object, the device including a detector operative for detecting light emitted or absorbed by the pH-sensing agent in response to the excitation light emitted by the light source; a first high-pass optical interference filter or a first dichroic mirror disposed over the detector, the first optical interference filter or the first dichroic mirror operative for allowing the light emitted by the pH-sensing agent to pass therethrough; and a second optical low-pass interference filter or a second dichroic mirror disposed between the light source and the surface of the device, the second optical interference filter or the second dichroic mirror operative for allowing the certain wavelengths of the excitation light emitted by the light source to pass therethrough and for reflecting light emitted by the pH-sensing agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification