Differential dissociation and melting curve peak detection
First Claim
1. A method for biological substance analysis, the method comprising:
- physically dissociating at least one sample of the biological substance by progressively changing a temperature of the at least one sample;
operating an emission detector to measure intensities of light emitted from a dye bound to the at least one sample as the temperature is progressively changed, thus producing measured intensities of light, wherein the dye remains bound to the at least one sample until the at least one sample is dissociated;
executing machine instructions by a system processor to cause the system to;
interpolate the measured intensities of light into a set of interpolated intensities for a plurality of temperatures at equally-spaced temperature intervals of the at least one sample including providing a user interface on a machine display to display a plurality of data points comprising the measured intensities of light at corresponding temperatures, the user interface also providing for marking of data points within a user-specified neighborhood or region, around or in proximity to a particular temperature of the plurality of temperatures and the machine instructions further causing the system to apply a weighting function to the marked data points to generate a new data sample value to be included in the set of interpolated intensities;
generate a differential dissociation curve from a derivative of the interpolated intensities;
determine a pedestal using one or more valleys proximate to a particular peak identified in the differential dissociation curve;
generate an adjusted differential dissociation curve by subtracting the pedestal from one or more peaks of the differential dissociation curve; and
generate the adjusted differential dissociation curve on a machine display.
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Accused Products
Abstract
Systems and methods are provided for processing a melting or dissociation curve of a DNA or other sample, for example, during PCR processing. In some embodiments, detection of the melting point and melting curve behavior can be enhanced by taking a derivative of the curve, and detecting peaks in the differential dissociation curve. In some embodiments, the derivative operation can comprise the use of edge-processing, or other detection algorithms. In some embodiments, the dissociation analysis can comprise removing low-frequency (or pedestal) components of the differential dissociation curve. In some embodiments, the differential dissociation curve can exhibit a smoothed or more regular appearance than the raw detected data.
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Citations
17 Claims
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1. A method for biological substance analysis, the method comprising:
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physically dissociating at least one sample of the biological substance by progressively changing a temperature of the at least one sample; operating an emission detector to measure intensities of light emitted from a dye bound to the at least one sample as the temperature is progressively changed, thus producing measured intensities of light, wherein the dye remains bound to the at least one sample until the at least one sample is dissociated; executing machine instructions by a system processor to cause the system to; interpolate the measured intensities of light into a set of interpolated intensities for a plurality of temperatures at equally-spaced temperature intervals of the at least one sample including providing a user interface on a machine display to display a plurality of data points comprising the measured intensities of light at corresponding temperatures, the user interface also providing for marking of data points within a user-specified neighborhood or region, around or in proximity to a particular temperature of the plurality of temperatures and the machine instructions further causing the system to apply a weighting function to the marked data points to generate a new data sample value to be included in the set of interpolated intensities; generate a differential dissociation curve from a derivative of the interpolated intensities; determine a pedestal using one or more valleys proximate to a particular peak identified in the differential dissociation curve; generate an adjusted differential dissociation curve by subtracting the pedestal from one or more peaks of the differential dissociation curve; and generate the adjusted differential dissociation curve on a machine display. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A system for generating a visualization of a differential dissociation curve of at least one sample on a machine display, comprising:
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an input unit for receiving, from sensors, emission measurements from at least one sample, the sample comprising a dye that remains bound to the sample until the sample is dissociated; and a processor, communicating with the input unit, the processor being configured to cause the system to; receive the emission measurements from the input unit and record the emission measurements at uneven temperature intervals of the sample; interpolate the emission measurements into a set of interpolated intensities for a plurality of temperatures at equally-spaced temperature intervals of the at least one sample including providing a on a machine display to display a plurality of data points comprising the emission measurements at corresponding temperatures, the user interface also providing for marking of data points within a user-specified neighborhood or region, around or in proximity to a particular temperature of the plurality of temperatures and the machine instructions further causing the system to apply a weighting function to the marked data points to generate a new data sample value to be included in the set of interpolated intensities; form a differential dissociation curve from a derivative of the set of interpolated intensities; determine a pedestal using one or more valleys proximate to a particular peak identified in the differential dissociation curve; generate an adjusted differential dissociation curve by subtracting the pedestal from one or more peaks of the differential dissociation curve; and form the adjusted differential dissociation curve on the machine display. - View Dependent Claims (9, 10, 11)
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12. A computer-readable medium, the computer-readable medium comprising instructions for a computer-implemented method for generating a differential dissociation curve of at least one sample on a machine display, the method comprising:
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interfacing with sensors to measure emission intensities of a dye bound to at least one sample, the emission intensities measured at uneven temperature intervals to produce emission measurements, wherein the dye remains bound to the at least one sample until the at least one sample is dissociated and wherein the at least one sample is dissociated by changing the temperature of the at least one sample; interpolate the emission measurements of the at least one sample into a set of interpolated intensities for a plurality of temperatures at equally-spaced temperature intervals of the at least one sample including providing a user interface on a machine display to display a plurality of data points comprising the emission measurements at corresponding temperatures, the user interface also providing for marking of data points within a user-specified neighborhood or region, around or in proximity to a particular temperature of the plurality of temperatures and the machine instructions further causing the processor to apply a weighting function to the marked data points to generate a new data sample value to be included in the set of interpolated intensities; generating a differential dissociation curve from a derivative of the interpolated intensities; determining a pedestal using one or more valleys proximate to a particular peak identified in the differential dissociation curve; generating an adjusted differential dissociation curve by subtracting the pedestal from one or more peaks of the differential dissociation curve; and generating the adjusted differential dissociation curve on the machine display. - View Dependent Claims (13, 14, 15, 16, 17)
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Specification