Methods of producing pancreatic hormones
First Claim
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1. A method for producing hormone secreting cells in vivo, said method comprising the steps of:
- a) producing human PDX-1 positive pancreatic hormone expressing cells in vitro; and
b) transplanting the human PDX-1 positive pancreatic hormone expressing cells into a mammalian subject, wherein the PDX-1 positive pancreatic hormone expressing cells secrete a hormone in vivo.
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Abstract
Disclosed herein are methods of producing pancreatic hormone-expressing cells by first differentiating pluripotent cells in cell culture so as to produce endodermal cells, the endodermal cells being competent to further differentiate into hormone-expressing cells capable of secreting at least one pancreatic hormone in response to a physiological signal, and then, transplanting the cultured endodermal cells into an organism, such as an organism in need of an endocrine cell therapy.
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Citations
30 Claims
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1. A method for producing hormone secreting cells in vivo, said method comprising the steps of:
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a) producing human PDX-1 positive pancreatic hormone expressing cells in vitro; and b) transplanting the human PDX-1 positive pancreatic hormone expressing cells into a mammalian subject, wherein the PDX-1 positive pancreatic hormone expressing cells secrete a hormone in vivo. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 23, 24)
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2. A method for producing hormone secreting cells, said method comprising the steps of:
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a) obtaining a population of human pluripotent stem cells; b) differentiating the population of step (a) into human definitive endoderm cells; c) differentiating the population of human definitive endoderm cells into a population of human PDX-1 expressing pancreatic endoderm cells; and d) transplanting the population of human PDX-1 expressing pancreatic endoderm cells into a mammal, wherein the transplanted cells of step (c) are matured into hormone producing pancreatic endocrine cells. - View Dependent Claims (17, 18, 19, 20, 21, 22, 25, 26, 27, 28, 29)
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30. The method of 28, wherein the pancreatic epithelium expands over time.
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