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Stabilized fibronectin domain compositions, methods and uses

  • US 9,982,253 B2
  • Filed: 12/15/2015
  • Issued: 05/29/2018
  • Est. Priority Date: 04/30/2010
  • Status: Active Grant
First Claim
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1. A method of generating a protein scaffold binding to a specific target with a predefined binding affinity, comprising the steps of:

  • a) contacting a library of a scaffold-based protein derived from a stability enhanced consensus sequence of a fibronectin type III domain incorporating randomized codons wherein a polynucleotide sequence encoding a polypeptide comprising a stability enhanced consensus sequence of a fibronectin type III domain of the amino acid sequence of SEQ ID NO;

    150 is provided, randomizing codons are introduced into the polynucleotide sequence at selected positions and copies of the polynucleotide sequence are propagated to form a library of polynucleotides encoding variant scaffold proteins, with the specific target; and

    b) isolating a protein scaffold binding to the specific target with the predefined affinity.

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