Methods for controlling pests using RNAi

• US 9,982,258 B2
• Filed: 09/12/2014
• Issued: 05/29/2018
• Est. Priority Date: 09/16/2005
• Status: Expired due to Fees

• Current Assignee: deVGen NV (China National Chemical Corporation)
• Original Assignee: deVGen NV (China National Chemical Corporation)
• Inventors: Raemaekers, Romaan, Feldmann, Pascale, Plaetinck, Geert, Nooren, Irene, Van Bleu, Els, Pecqueur, Frederic, Kubler, Laurent, Damme, Nicole, Degrave, Lies, Remory, Isabel, Bogaert, Thierry
• Primary Examiner(s): Shin, Dana H
• Application Number:
  US14/485,103
• Publication Number:
  US 20150065557A1
• Time in Patent Office: 1,355 Days
• Field of Search: None
• CPC Class Codes: A01N 63/60 Isolated nucleic acids
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention relates to methods for controlling pest infestation using double stranded RNA molecules. The invention provides methods for producing transgenic cells expressing the double stranded RNA molecules, as well as compositions and commodity products containing or treated with such molecules.

Claims

1. An isolated double stranded ribonucleotide (RNA) molecule, wherein the RNA molecule is a pest control agent targeting a gene encoding beta-coatomer, wherein the RNA molecule comprises complementary strands, wherein one strand of the RNA molecule is produced from the expression of a polynucleotide molecule selected from the group consisting of:

(a) a polynucleotide molecule comprising the nucleic acid sequence set forth in SEQ ID NO;

23, or a fragment of at least 21 nucleotides thereof; and

(b) a polynucleotide molecule comprising a nucleic acid sequence at least 85% identical to SEQ ID NO;

23,

wherein ingestion of said RNA molecule by a pest results in down-regulation of the target gene in the pest.

2. A cell transformed with the RNA molecule of claim 1.

3. The cell of claim 2, wherein said cell is a bacterial, yeast, or algal cell.

4. A food product comprising the cell of claim 3.

5. A composition comprising the double stranded ribonucleotide molecule of claim 1, wherein said composition is selected from the group consisting of a spray, powder, pellet, gel, and capsule.

6. A method for controlling pest infestation, comprising exposing a pest to a composition comprising the double stranded ribonucleotide molecule of claim 1.

7. A pesticide comprising the double stranded ribonucleotide molecule of claim 1, wherein the pesticide is any substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest.

8. A method for protecting an object from pest infestation, comprising treating the surface of said object with a composition comprising the double stranded ribonucleotide molecule of claim 1.

9. The method of claim 8, wherein said object is selected from the group consisting of wood, tree, book binding, cloth, and a food storage container.

10. A vector encoding the double stranded RNA molecule of claim 1.