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Polony sequencing methods

  • US 9,982,296 B2
  • Filed: 09/30/2015
  • Issued: 05/29/2018
  • Est. Priority Date: 10/09/2009
  • Status: Active Grant
First Claim
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1. A method for obtaining the sequence of a polynucleotide comprising an unknown sequence, the method comprising:

  • providing a polynucleotide library comprising a plurality of polynucleotide fragments comprising unknown polynucleotide sequences;

    providing a substrate comprising a set of polynucleotide fragments having known nucleotide sequences from a reference genome;

    sequencing the ends of the library of polynucleotide fragments, thereby creating partially-sequenced fragments;

    comparing the sequenced ends of the partially-sequenced fragments to the reference genome;

    sequencing the rest of the partially-sequenced fragments, wherein sequencing the partially-sequenced fragments comprises;

    contacting at least a portion of the partially-sequenced fragments with the substrate under conditions suitable to allow the partially-sequenced fragments to hybridize with a complementary substrate polynucleotide fragment; and

    detecting the hybridizations; and

    obtaining the sequence of the entire partially-sequenced fragments from the known polynucleotide sequence of reference genome to which the partially-sequenced fragments hybridize.

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