Methods and compositions for enhancing immunoassays
First Claim
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1. A method of detecting interleukin-2 (IL-2) comprising:
- (a) obtaining a fluidic cell comprising an optical ring resonator having a first anti-cytokine antibody attached thereto, wherein the first anti-cytokine antibody is anti-IL-2 from clone 555051 antibody;
(b) contacting the first anti-cytokine antibody with a sample comprising IL-2 that selectively binds to the anti-cytokine antibody;
(c) contacting the bound IL-2 with a second anti-cytokine antibody that selectively binds to the IL-2, wherein the second anti-cytokine antibody is a biotinylated anti-IL-2 from clone 555040 antibody;
(d) contacting the biotinylated second anti-cytokine antibody with a streptavidin-horseradish peroxidase conjugate;
(e) contacting the horseradish peroxidase with a substantially continuous flow of a solution comprising 4-chloro-1-naphthol and hydrogen peroxide, under conditions that oxidize 4-chloro-1-naphthol to 4-chloro-1-naphthon, whereby the 4-chloro-1-naphthon precipitates on the surface of the optical ring resonator during the substantially continuous flow of the solution; and
(f) measuring a change in resonance wavelengths of the optical ring resonator, thereby indicating the presence of the IL-2.
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Abstract
Embodiments of the methods, compositions, and systems provided herein relate to enzymatic enhancement of immunoassays using photonic sensor arrays.
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Citations
10 Claims
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1. A method of detecting interleukin-2 (IL-2) comprising:
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(a) obtaining a fluidic cell comprising an optical ring resonator having a first anti-cytokine antibody attached thereto, wherein the first anti-cytokine antibody is anti-IL-2 from clone 555051 antibody; (b) contacting the first anti-cytokine antibody with a sample comprising IL-2 that selectively binds to the anti-cytokine antibody; (c) contacting the bound IL-2 with a second anti-cytokine antibody that selectively binds to the IL-2, wherein the second anti-cytokine antibody is a biotinylated anti-IL-2 from clone 555040 antibody; (d) contacting the biotinylated second anti-cytokine antibody with a streptavidin-horseradish peroxidase conjugate; (e) contacting the horseradish peroxidase with a substantially continuous flow of a solution comprising 4-chloro-1-naphthol and hydrogen peroxide, under conditions that oxidize 4-chloro-1-naphthol to 4-chloro-1-naphthon, whereby the 4-chloro-1-naphthon precipitates on the surface of the optical ring resonator during the substantially continuous flow of the solution; and (f) measuring a change in resonance wavelengths of the optical ring resonator, thereby indicating the presence of the IL-2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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