Method and apparatus for use in temperature controlled processing of microfluidic samples

• US 9,987,636 B2
• Filed: 04/20/2015
• Issued: 06/05/2018
• Est. Priority Date: 04/04/2005
• Status: Active Grant

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Jensen, Morten J, Chow, Andrea W, Kennedy, Colin B., Mouradian, Stephane
• Primary Examiner(s): Priest, Aaron A
• Application Number:
  US14/691,340
• Publication Number:
  US 20150224505A1
• Time in Patent Office: 1,142 Days
• Field of Search: None
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: B01L 7/5255 by moving sample containers

Abstract

Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.

Claims

1. An apparatus for use with a microfluidic device, the apparatus comprising:

a plurality of thermal elements, each of the plurality of thermal elements being temperature controlled;

a thermal element tray operable to mount the plurality of thermal elements, the plurality of thermal elements being mounted parallel to each other;

a drive operable to move the microfluidic device laterally relative to the thermal element tray and align one of the plurality of thermal elements with a heating area of the microfluidic device; and

a lift operable to move the aligned one of the plurality of thermal elements tangentially relative to the microfluidic device to contact the heating area.

2. The apparatus of claim 1, wherein the thermal elements comprise a plurality of bars.

3. The apparatus of claim 2, wherein the drive is operably connected to control the lift.

4. The apparatus of claim 1, wherein the lift includes:

a rotatable shaft; and

a plurality of bars radially mounted about the rotatable shaft;

wherein the lift is further operable to move one of the plurality of bars to contact the aligned one of the plurality of thermal elements.

5. The apparatus of claim 1, wherein the lift includes a linear actuator operable to move the aligned one of the plurality of thermal elements.

6. The apparatus of claim 1, further comprising a processor responsive to a protocol application to control positions of and temperatures of each of the plurality of thermal elements.

7. The apparatus of claim 1, wherein each of the plurality of thermal elements is selected from the group consisting of a resistive element and a Peltier device.

8. The apparatus of claim 1, wherein at least one of the drive and the lift is operable to position one of the plurality of thermal elements and the microfluidic device such that the one of the plurality of thermal elements contacts the top of the microfluidic device.

9. The apparatus of claim 1, wherein at least one of the drive and the lift is operable to position one of the plurality of thermal elements and the microfluidic device such that the one of the plurality of thermal elements contacts the bottom of the microfluidic device.

10. The apparatus of claim 1, wherein at least one of the drive and the lift is operable to position one of the plurality of thermal elements above the microfluidic device and the microfluidic device such that the one of the plurality of thermal elements above the microfluidic device contacts the top and the bottom of the microfluidic device, and to position one of the plurality of thermal elements below the microfluidic device and the microfluidic device such that the one of the plurality of thermal elements below the microfluidic device contacts the bottom of the microfluidic device.

11. The apparatus of claim 6, wherein the processor is further responsive to the protocol application to thermally ramp at least one of the plurality of thermal elements when the at least one of the plurality of thermal elements contacts the heating area.

12. The apparatus of claim 6, wherein the processor is further responsive to the protocol application to break contact between at least one of the plurality of thermal elements and the heating area.

13. The apparatus of claim 12, wherein the processor is further responsive to the protocol application to thermally ramp at least one of the plurality of thermal elements during the respective period of time the at least one of the plurality of thermal elements contacts the microfluidic device.

14. The apparatus of claim 1, further comprising an interface material imposed between the aligned one of the plurality of thermal elements and the heating area when the aligned one of the plurality of thermal elements contacts the heating area.

15. The apparatus of claim 14, wherein the interface material is selected from the group consisting of a wax, a polymer, and a thermally conductive insulator material.

16. The apparatus of claim 6 wherein the processor is further responsive to the protocol application to successively contact each of the plurality of thermal elements with the heating area and to control temperature for one of the plurality of thermal elements in contact with the heating area to bring temperature of the heating area to a predetermined temperature for a predetermined time.