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Covalent modification of biological macromolecules

  • US 9,988,433 B2
  • Filed: 03/13/2014
  • Issued: 06/05/2018
  • Est. Priority Date: 03/14/2013
  • Status: Active Grant
First Claim
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1. A method of covalently modifying a fibrinogen protein, comprising:

  • (I) subjecting a fibrinogen protein to denaturing and reducing conditions to produce a reduced, denatured fibrinogen protein comprising one or more thiol groups;

    (II) reacting the reduced, denatured fibrinogen protein with one or more norbornene moieties underconditions sufficient to produce a photoinitiated radical thiol-ene coupling reaction between at least one of the one or more norbornene moieties and at least one of the one or more thiol groups on the reduced, denatured fibrinogen protein, thereby providing a covalently modified fibrinogen protein,wherein the one or more norbornene moieties comprise a polymer selected from the group consisting of;

    poly(lactic acid) (PLA), polyglycolide (PGA), copolymer of PLA and PGA (PLGA), poly(vinyl alcohol) (PVA), poly(ethylene glycol) (PEG), poly(ethylene oxide), poly(ethylene oxide)-co-poly(propylene oxide) block copolymer, poloxamine, polyanhydride, polyorthoester, poly(hydroxy acid), polydioxanone, polycarbonate, polyaminocarbonate, poly(vinyl pyrrolidone), poly(ethyl oxazoline), carboxymethyl cellulose, and hydroxyalkylated cellulose.

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