Fiber sampler for recovery of bioaerosols and particles

• US 9,988,664 B2
• Filed: 11/24/2015
• Issued: 06/05/2018
• Est. Priority Date: 04/08/2004
• Status: Active Grant

• Current Assignee: Research Triangle Institute
• Original Assignee: Research Triangle Institute
• Inventors: Ensor, David Samuel, Walls, Howard Jerome, Foarde, Karin K
• Primary Examiner(s): Hixson, Christopher Adam
• Assistant Examiner(s): Berkeley, Emily R.
• Application Number:
  US14/950,813
• Publication Number:
  US 20160076073A1
• Time in Patent Office: 924 Days
• Field of Search: 436178
• CPC Class Codes: B01D 2239/025 comprising nanofibres apparatus incorporating such gas filtering material see B01D46/546
• CPC Class Codes: B01D 2239/0604 Arrangement of the fibres in the filtering material
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
• CPC Class Codes: G01N 1/2205 with filters
• CPC Class Codes: G01N 1/2208 with impactors
• CPC Class Codes: G01N 1/2214 by sorption
• CPC Class Codes: G01N 2001/2223 aerosol sampling devices
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath

Abstract

A bioparticle collection device and an aerosol collection system. The bioparticle collection device includes a collection medium including a plurality of fibers formed into a fiber mat and configured to collect bioparticles thereon, and includes a viability enhancing material provider disposed in a vicinity of the plurality of fibers and configured to provide a viability enhancing material to the collected bioparticles to maintain viability of the bioparticles collected by the fiber mat. The aerosol collection system includes an aerosol pumping device configured to entrain particles in an gas stream, an aerosol saturation device configured to saturate the particles in the gas stream with a biocompatible liquid, and an aerosol collection medium downstream from the aerosol saturation device and including a plurality of fibers formed into a fiber mat for collection of the saturated aerosol particles.

Claims

1. A method for collecting aerosols including bioparticles, comprising:

entraining the aerosols including the bioparticles in a gas stream;

saturating the bioparticles in the gas stream with a biocompatible liquid;

collecting the saturated bioparticles by a collection medium including a plurality of fibers of a fiber mat including a viability enhancing material; and

maintaining viability of the bioparticles while in the fiber mat both during and after collection of the bioparticles with the fiber mat such that the bioparticles collected in the fiber mat are capable of becoming active again after being placed into a favorable environment.

2. The method of claim 1, further comprising providing the viability enhancing material into the collection medium prior to collecting the bioparticles.

3. The method of claim 2, wherein the providing comprises providing at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth.

4. The method of claim 1, further comprising providing the viability enhancing material into the collection medium during the collecting of the aerosol particles.

5. The method of claim 4, wherein the providing comprises providing at least one of water, proteins, carbohydrates, sugars, salts, phosphate buffered saline, and tryptic soy broth.

6. The method of claim 1, further comprising introducing antioxidant gases into the collection medium.

7. The method of claim 1, further comprising

providing a liquid to the fiber mat.

8. The method of claim 1, wherein collecting comprises collecting the bioparticles in said collection medium comprising at least one of polysulfone, polyurethane, nylon, polycaprolacton, and polystyrene.

9. The method of claim 7, wherein collecting comprises collecting the bioparticles in said collection medium comprising at least one of polysulfone, polyurethane, nylon, polycaprolacton, and polystyrene.

10. The method of claim 1, wherein collecting comprises collecting the bioparticles in said collection medium comprising a graphite coated fiberweb support supporting said fiber mat.

11. The method of claim 7, wherein collecting comprises collecting the bioparticles in said collection medium comprising a graphite coated fiberweb support supporting said fiber mat.

12. The method of claim 1, wherein collecting comprises collecting the bioparticles in said collection medium being provided with a nutrient supply of tryptic soy broth.

13. The method of claim 7, wherein collecting comprises collecting the bioparticles in said collection medium being provided with a nutrient supply of tryptic soy broth.

14. The method of claim 1, wherein collecting comprises collecting the bioparticles in said collection medium controlled at a relative humidity between 65 to 85%.

15. The method of claim 7, wherein collecting comprises collecting the bioparticles in said collection medium controlled at a relative humidity between 65 to 85%.

16. The method of claim 1, further comprising storing the fiber mat after collection of the bioparticles in a relative humidity controlled environment.

17. The method of claim 7, further comprising storing the fiber mat after collection of the bioparticles in a relative humidity controlled environment.

18. The method of claim 1, wherein collecting comprises providing to the collection medium agents to reduce oxygen toxicity.

19. The method of claim 18, wherein providing agents comprises providing at least one of enzymes or fullerenes to reduce said oxygen toxicity.

20. The method of claim 7, wherein collecting comprises providing to the collection medium agents to reduce oxygen toxicity.