Apparatus for amplification of nucleic acids
First Claim
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1. An apparatus for analyzing a biological sample comprising:
- a) a semiconductor substrate comprising an integrated circuitry,wherein the integrated circuitry on the semiconductor substrate comprises;
a junction temperature sensor; and
a first light sensor;
b) a biocompatible coating over the semiconductor substrate,wherein the biocompatible coating is positioned to allow the biological sample to be in proximity to the integrated circuitry;
c) one or more processors;
d) memory storing one or more programs, the one or more programs configured to be executed by the one or more processors, the one or more programs including instructions for;
detecting a first light input measurement from the first light sensor at a first time prior to triggering a light signal from the biological sample;
detecting a second light input measurement from the first light sensor at a second time after triggering the light signal from the biological sample;
detecting a plurality of temperature input measurements from the junction temperature sensor,wherein the plurality of temperature input measurements comprises;
a first temperature input measurement detected at the first time; and
a second temperature input measurement detected at the second time;
using the first light measurement to estimate noise signal; and
using the plurality of temperature input measurements to further estimate noise signal and improve noise suppression, thereby determining the light signal from the biological sample.
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Abstract
Described herein is a chip-based apparatus for amplifying nucleic acids, a cartridge housing the apparatus, and methods of using the apparatus for amplification of nucleic acids. More specifically, this invention provides integrated semiconductor chip, manufactured with standard semiconductor manufacturing process, with on-chip circuitry to perform thermal management and optical sensing necessary for amplification of nucleic acids. The apparatus and methods embodied in this invention makes it possible to build a disease diagnosis and prognosis tool that is easy to use, portable and disposable.
28 Citations
36 Claims
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1. An apparatus for analyzing a biological sample comprising:
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a) a semiconductor substrate comprising an integrated circuitry, wherein the integrated circuitry on the semiconductor substrate comprises; a junction temperature sensor; and a first light sensor; b) a biocompatible coating over the semiconductor substrate, wherein the biocompatible coating is positioned to allow the biological sample to be in proximity to the integrated circuitry; c) one or more processors; d) memory storing one or more programs, the one or more programs configured to be executed by the one or more processors, the one or more programs including instructions for; detecting a first light input measurement from the first light sensor at a first time prior to triggering a light signal from the biological sample; detecting a second light input measurement from the first light sensor at a second time after triggering the light signal from the biological sample; detecting a plurality of temperature input measurements from the junction temperature sensor, wherein the plurality of temperature input measurements comprises; a first temperature input measurement detected at the first time; and a second temperature input measurement detected at the second time; using the first light measurement to estimate noise signal; and using the plurality of temperature input measurements to further estimate noise signal and improve noise suppression, thereby determining the light signal from the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification