Nucleic acid production and sequence analysis
First Claim
1. A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer, wherein the linking unit is attached with a covalent linkage to a target site in the template nucleic acid sequence, wherein the linking unit comprises a primer binding region to which the primer is base paired and a linking region between the primer binding region and the covalent linkage, wherein the linking region does not base pair with the primer or with the nucleic acid molecule that is produced.
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Abstract
A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage.
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Citations
36 Claims
- 1. A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer, wherein the linking unit is attached with a covalent linkage to a target site in the template nucleic acid sequence, wherein the linking unit comprises a primer binding region to which the primer is base paired and a linking region between the primer binding region and the covalent linkage, wherein the linking region does not base pair with the primer or with the nucleic acid molecule that is produced.
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31. A template nucleic acid sequence attached to a linking unit and a primer, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage, and wherein the 3′
- end of the primer is attached to the linking unit.
Specification