Sample processing improvements for microscopy

• US 9,989,750 B2
• Filed: 11/23/2016
• Issued: 06/05/2018
• Est. Priority Date: 06/26/2013
• Status: Active Grant

• Current Assignee: Alentic Microscience Inc.
• Original Assignee: Alentic Microscience Inc.
• Inventors: Fine, Alan Marc, Macaulay, Hershel, Hymes-Vandermeulen, Noah
• Primary Examiner(s): Punnoose, Roy M
• Application Number:
  US15/360,724
• Publication Number:
  US 20170075099A1
• Time in Patent Office: 559 Days
• Field of Search: 356244, 356 36
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 15/0606 by collecting particles on a support
• CPC Class Codes: G01N 15/0612 Optical scan of the deposits G01N15/0625 takes precedence
• CPC Class Codes: G01N 15/1468 with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/1029 Particle size
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 21/59 Transmissivity G01N21/25 takes precedence
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

Claims

1. An apparatus comprising

a two-dimensional arrangement of light sensitive elements exposed at a sensor surface of an imaging sensor,

a second surface to be moved into a predefined position relative to the imaging surface to form a sample space that is between the sensor surface and the second surface and contains at least part of a sample, and

a mechanism configured to cause mixing of the sample by moving the second surface toward and away from the sensor surface repeatedly.

2. The apparatus of claim 1 in which the mechanism moves the second surface at a controlled velocity.

3. The apparatus of claim 1 in which the mechanism comprises an actuator.

4. The apparatus of claim 3 in which the actuator comprises a pump.

5. The apparatus of claim 1 in which the mechanism is configured to move the second surface toward and away from the sensor surface without reaching the predefined position.

6. The apparatus of claim 1 in which the mixing of the sample comprises causing sample units to be evenly distributed in the sample.

7. The apparatus of claim 1 in which the mechanism is automatically controlled.

8. The apparatus of claim 1 in which the mechanism is configured to move the second surface along a trajectory.

9. The apparatus of claim 1 in which the trajectory comprises an arc.

10. The apparatus of claim 1 in which the sample comprises elements that are to be counted, and the mechanism is configured so that the moving of the second surface causes the elements to be evenly distributed across the sensor surface and causes the bulk concentration of the elements in the sample after the second surface reaches the predefined position to be consistently proportional to the bulk concentration of the elements in the sample when the second surface is in an initial position.

11. The apparatus of claim 1 in which the bulk concentration of elements in the sample after the second surface reaches the predefined position is the same as or higher than the bulk concentration of the elements in the sample when the second surface is in an initial position.

12. The apparatus of claim 1 in which the second surface has an alignment edge that bears against an alignment edge associated with the sensor surface to define a pivot axis about which the second surface is to be rotated to reach the predefined position.

13. A method comprising

repeatedly moving a first surface toward and away from a sensor surface of an imaging sensor,

then moving the first surface to a predetermined position relative to the sensor surface, and

using the imaging sensor to capture an image of a sample in a space between the first surface and the sensor surface when the first surface is at the predetermined position.

14. The method of claim 13 comprising providing the sample in a space between the first surface and the second surface before the imaging sensor captures the image.

15. The method of claim 14 in which providing the sample comprises enabling capillary flow to move into the sample into the space.

16. The method of claim 13 in which moving the first surface to the predetermined position relative to the sensor surface comprises forcing the sample against the sensor surface.

17. The method of claim 13 in which at least one of the repeated moving or the moving of the first surface to the predetermined position is controlled automatically.

18. The method of claim 13 comprising ejecting the sample into the space before repeatedly moving the first surface toward and away from the sensor surface.

19. The method of claim 18 in which the sample is ejected automatically.