Detecting and visualizing correlations between measured correlation values and correlation reference values of a pathway
First Claim
1. A method involving a correlation value determining and visualization system, the method comprising:
- (a) segmenting a digital image of a tissue slice of a cancer patient into first image objects representing immune cells and second image objects representing cancer cells, wherein the tissue slice was stained with a first antibody that is specific to a first protein present in immune cells, and wherein the tissue slice was stained with a second antibody that is specific to a second protein present in cancer cells;
(b) identifying a reference correlation value from among a plurality of reference correlation values indicative of protein-protein interactions in biochemical pathways, wherein the reference correlation value is indicative of a correlation between immune cells and cancer cells;
(c) identifying an image region in the digital image, wherein the image region is divided into a set of tiles;
(d) generating, for each tile, a first value and a second value, wherein the first value is indicative of a density of the first image objects in the tile, and wherein the second value is indicative of a density of the second image objects in the tile;
(e) determining, for the image region, a measured correlation value that is indicative of a relationship between the first value and the second value;
(f) comparing the measured correlation value determined in (e) with the reference correlation value identified in (b) and thereby generating a correspondence value; and
(g) displaying the image region on a display of the system along with an associated visual indication representing the correspondence value determined in (f), wherein (a) through (g) are performed by the system.
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Abstract
An analysis and visualization system analyzes a digital image of a tissue sample. In the sample, cells of a first type are stained in a first way, and cells of a second type are stained in a second way. The system segments the high-resolution image into first and second objects representing cells of the first and second types, respectively. The system also identifies a region of interest, and divides it into tiles. The system generates, for each tile, a first value and a second value. The first and second values for a tile are indicative of densities of the first and second objects in the tile. From the values, a measured correlation coefficient (CC) value is determined. The system compares the measured CC value to a reference CC value, thereby obtaining a correspondence value. The system then displays the image region along with a visualization of the correspondence value.
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Citations
21 Claims
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1. A method involving a correlation value determining and visualization system, the method comprising:
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(a) segmenting a digital image of a tissue slice of a cancer patient into first image objects representing immune cells and second image objects representing cancer cells, wherein the tissue slice was stained with a first antibody that is specific to a first protein present in immune cells, and wherein the tissue slice was stained with a second antibody that is specific to a second protein present in cancer cells; (b) identifying a reference correlation value from among a plurality of reference correlation values indicative of protein-protein interactions in biochemical pathways, wherein the reference correlation value is indicative of a correlation between immune cells and cancer cells; (c) identifying an image region in the digital image, wherein the image region is divided into a set of tiles; (d) generating, for each tile, a first value and a second value, wherein the first value is indicative of a density of the first image objects in the tile, and wherein the second value is indicative of a density of the second image objects in the tile; (e) determining, for the image region, a measured correlation value that is indicative of a relationship between the first value and the second value; (f) comparing the measured correlation value determined in (e) with the reference correlation value identified in (b) and thereby generating a correspondence value; and (g) displaying the image region on a display of the system along with an associated visual indication representing the correspondence value determined in (f), wherein (a) through (g) are performed by the system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 21)
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14. A method involving a correlation value determining and visualization system, the method comprising:
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(a) receiving a digital image of a tissue sample of a cancer patient into the system, wherein the tissue sample includes first image objects stained with a first antibody stain, and wherein the tissue sample also includes second image objects stained with a second antibody stain; (b) identifying in the digital image the first image objects; (c) identifying in the digital image the second image objects; (d) identifying a reference correlation value from among a plurality of reference correlation values indicative of protein-protein interactions in biochemical pathways, wherein the reference correlation value is indicative of a correlation between the first image objects and the second image objects; (e) identifying an image region in the digital image, wherein the image region is divided into a set of tiles; (f) generating, for each tile of the image region, a first value and a second value, wherein the first value is indicative of a density of the first image objects in the tile, and wherein the second value is indicative of a density of the second image objects in the tile; (g) determining a measured correlation value indicative of a relationship between the first value and the second value; (h) comparing the measured correlation value determined in (g) with the reference correlation value identified in (d) thereby generating a correspondence value; and (i) displaying the image region on a display of the system along with an associated visual indication representing the correspondence value determined in (h), wherein (a) through (h) are performed by the system. - View Dependent Claims (15, 16, 17)
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18. A method involving a correlation value determining and visualization system, the method comprising:
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(a) storing digital image information, of a tissue sample of a cancer patient on the system, wherein the tissue sample includes first image objects stained with a first stain, and wherein the tissue sample also includes second image objects stained with a second stain; (b) storing a plurality of reference correlation values on the system that indicate how specified proteins correlate with other proteins in biochemical pathways, wherein one of the plurality of reference correlation values is indicative of a correlation between objects stained with the first stain and objects stained with the second stain; (c) storing a plurality of measured correlation values on the system, wherein one of the measured correlation values is indicative of a correlation between the first image objects and the second image objects, wherein said one measured correlation value was generated by the system from the digital image information; (d) receiving user input information into the system, wherein the user input information selects a biochemical pathway; and (e) in response to the receiving in (d) causing a visual indication of a correspondence value to be displayed on a display of the system, wherein the correspondence value is indicative of a comparison of said one measured correlation value with said one of the plurality of reference correlation values, and wherein (a) through (e) are performed by the system. - View Dependent Claims (19, 20)
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Specification