×

Single molecule loading methods and compositions

  • US 9,993,794 B2
  • Filed: 11/04/2014
  • Issued: 06/12/2018
  • Est. Priority Date: 03/31/2008
  • Status: Active Grant
First Claim
Patent Images

1. A method of distributing single polymerase enzyme molecules into a plurality of array regions comprising:

  • providing a surface comprising the plurality of array regions, each array region comprising several binding elements; and

    exposing the surface to a solution comprising polymerase enzymes, each polymerase enzyme bound to a binding structure comprising several functional moieties, whereby the functional moieties of the binding structure react with the binding elements on the array regions; and

    whereby in at least some of the array regions, the functional moieties on the binding structure react with other available binding sites in a given array region, depleting the binding sites and preventing other polymerase-binding structures from loading, resulting in a single polymerase enzyme bound to each of these regions.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×