Catalyst-free surface functionalization and polymer grafting
First Claim
1. A substrate comprising a first surface comprising silane or a silane derivative covalently bound, optionally through a linker, to a functionalized molecule through reaction of the functionalized molecule with a first plurality of unsaturated moieties selected from the group consisting of cycloalkenes, heterocycloalkenes, and optionally substituted variants and combination thereof covalently attached to said silane or silane derivative, wherein the functionalized molecule comprises a hydrogel or a polymer that comprises a recurring unit of Formula (I) and a recurring unit of Formula (II):
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Abstract
Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.
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Citations
28 Claims
- 1. A substrate comprising a first surface comprising silane or a silane derivative covalently bound, optionally through a linker, to a functionalized molecule through reaction of the functionalized molecule with a first plurality of unsaturated moieties selected from the group consisting of cycloalkenes, heterocycloalkenes, and optionally substituted variants and combination thereof covalently attached to said silane or silane derivative, wherein the functionalized molecule comprises a hydrogel or a polymer that comprises a recurring unit of Formula (I) and a recurring unit of Formula (II):
Specification