Animal meal that includes fungal biomass

• US 9,994,804 B2
• Filed: 04/07/2014
• Issued: 06/12/2018
• Est. Priority Date: 05/07/2010
• Status: Active Grant

• Current Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Original Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Inventors: Menon, Suresh M., Suresh, Arul Victor, Orchard, Samantha S., Alisala, Kashinatham, Sircar, Jagadish C., Hash, Sara Ingegard
• Primary Examiner(s): Kashnikow, Erik
• Assistant Examiner(s): Zilbering, Assaf
• Application Number:
  US14/247,134
• Publication Number:
  US 20140363536A1
• Time in Patent Office: 1,527 Days
• Field of Search: 426 2, 426583, 426 49, 426 61
• CPC Class Codes: A01N 63/36 Penicillium
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 20/142 Amino acids; Derivatives thereof
• CPC Class Codes: A23K 20/147 Polymeric derivatives, e.g. peptides or proteins
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 40/20 by moulding, e.g. making cakes or briquettes
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/42 Dry feed
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 33/10 using additives addition of substantially indigestible substances A23L33/21
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11C 3/003 by esterification of fatty acids with alcohols C11C3/02 takes precedence
• CPC Class Codes: C12M 21/12 for producing fuels or solvents C12M21/04 takes precedence; liquid carbonaceous fuels C10L1/00, solid fuels C10L5/00
• CPC Class Codes: C12M 25/02 Membranes; Filters filters or filtration in general B01D24/00-B01D41/00
• CPC Class Codes: C12M 27/02 : Stirrer or mobile mixing elements
• CPC Class Codes: C12M 27/20 : Baffles; Ribs; Ribbons; Auger vanes
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 : Fungal isolates
• CPC Class Codes: C12P 2203/00 : Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 5/005 : aromatic naphthacene C12P29/00
• CPC Class Codes: C12P 5/02 : acyclic C12P5/007 takes precedence
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6481 : Phosphoglycerides phosphoglycerides having carboxylic acids with less than seven carbon atoms C12P7/62
• CPC Class Codes: C12R 2001/645 : Fungi ; Processes using fungi
• CPC Class Codes: C12R 2001/80 : Penicillium
• CPC Class Codes: Y02A 40/818 : Alternative feeds for fish, e.g. in aquacultures
• CPC Class Codes: Y10S 426/805 : Pet food for dog, cat, bird, or fish
• CPC Class Codes: Y10S 426/807 : Poultry or ruminant feed

Abstract

Disclosed herein are compositions comprising an isolated cellulose degrading fungus. Also disclosed are culture compositions and bioreactor compositions comprising the cellulose degrading fungus. Further described herein are filtration and extraction devices comprising the cellulose degrading fungus. Still further disclosed are bioprocessing facilities for and methods for producing co-products resulting from one or more bioprocesses of the cellulose degrading fungus.

Claims

1. A method of manufacture of an animal feed comprising combining dried fungal biomass with whey, thereby forming an admixture, wherein the admixture is suitable for animal consumption, thereby manufacturing an animal feed, wherein the dried fungal biomass comprises a fungus selected from the group consisting of:

a fungus having a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequence identity to the nucleic acid of SEQ ID NO;

1;

a fungus having an ITS1 sequence with at least 98% sequence identity to SEQ ID NO;

2;

a fungus having an ITS2 sequence with at least 98% sequence identity to SEQ ID NO;

3;

a fungus having a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO;

4, Penicillium menonorum and a species being the same as NRRL deposit Accession No;

50410.

2. The method of claim 1, wherein the dried fungal biomass comprises a fungus selected from the group consisting of:

a fungus having a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequence identity to the nucleic acid of SEQ ID NO;

1;

a fungus having an ITS1 sequence with at least 98% sequence identity to SEQ ID NO;

2;

a fungus having an ITS2 sequence with at least 98% sequence identity to SEQ ID NO;

3; and

a fungus having a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO;

4.

3. The method of claim 1, wherein the dried fungal biomass comprises a fungus that is the same as NRRL deposit Accession No:

50410.

4. The method of claim 1, wherein the admixture is in the form of a slurry.

5. The method of claim 1, wherein the fungal biomass is produced by a method comprising:

a) inoculating a medium with the fungus;

b) fermenting the inoculated medium under aerobic conditions, thereby producing a fungal biomass;

c) recovering the fungal biomass; and

d) drying the fungal biomass, thereby producing dried fungal biomass.

6. The method of claim 5, wherein the medium comprises biological matter.

7. The method of claim 6, wherein the biological matter comprises at least one of:

simple sugars, cane juice or its condensates or its dry solid evaporate, whey, grains, stover, forage, grasses, oilseed crops, nut shells, nut hulls, fruit pomace, plant waste, algae, wood, wood byproducts, bark, paper, paper products, animal manure, food waste, yard waste, almond hulls, grape pomace, agriculture waste, and sawdust.

8. The method of claim 6, wherein the biological matter comprises at least one of:

a cellulosic carbon source or a lysate or a hydrolysate thereof, a hemicellulosic carbon source or a lysate or a hydrolysate thereof, and a lignocellulosic carbon source or a lysate or a hydrolysate thereof.

9. The method of claim 6, further comprising extracting a lipid product from fungal biomass prior to drying.

10. The method of claim 9, wherein the lipid product comprises at least one of:

triacylglyceride, wax esters, mono-unsaturated hydrocarbon chains, di-unsaturated hydrocarbon chains, and any molecular forms having a straight-chain saturated hydrocarbon portion.

11. The method of claim 9, wherein the fungal biomass has been substantially depleted of a lipid product.

12. The method of claim 1, wherein said admixture has dry fungal biomass/whey ratio ranging from about 1:

100 to about 100;

1 (vol/vol).

13. The method of claim 12, wherein said admixture has dry fungal biomass/whey ratio ranging from about 1:

10 to about 10;

1 (vol/vol).

14. The method of claim 12, wherein said admixture has dry fungal biomass/whey ratio ranging from about 1:

3 to about 1;

7 (vol/vol).

15. The method of claim 1, wherein said admixture comprises 5% to 20% fungal biomass by weight.

16. The method of claim 1, wherein the animal feed is formulated for a land animal.

17. The method of claim 1, wherein the fungal biomass comprises greater than 25% cell dry weight (cdw) crude protein.

18. The method of claim 1, wherein the fungal biomass comprises between 1% and 90% of the admixture by weight.

19. The method of claim 1, wherein the fungal biomass comprises between 80% and 95% of the admixture by weight.

20. The method of claim 1, wherein the fungal biomass comprises at least 10% (cdw) lipid comprising triacylglycerol (TAG).

21. The method of claim 1, wherein the fungal biomass comprises biomass harvested at either a time that an available nitrogen ingredient is exhausted, or a time when log-phase growth terminates.

22. The method of claim 1, wherein said fungus has a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequencing identity to the nucleic acid of SEQ ID NO:

1.

23. The method of claim 1, wherein said fungus has a 5.8S ribosomal RNA gene sequence of SEQ ID NO:

1.

24. The method of claim 1, wherein said fungus has an ITS1 sequence with at least 98% sequence identity to SEQ ID NO:

2.

25. The method of claim 1, wherein said fungus has an ITS1 sequence of SEQ ID NO:

2.

26. The method of claim 1, wherein said fungus has an ITS2 sequence with at least 98% sequence identity to SEQ ID NO:

3.

27. The method of claim 1, wherein said fungus has an ITS2 sequence of SEQ ID NO:

3.

28. The method of claim 1, wherein said fungus has a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO:

4.

29. The method of claim 1, wherein said fungus has a 28S ribosomal RNA gene sequence of SEQ ID NO:

4.

30. The method of claim 1, wherein the fungus is Penicillium menonorum.

31. The method of claim 1, wherein the fungal biomass comprises about 25% to 75% (cdw) crude protein.

32. The method of claim 1, wherein the admixture is in the form of a pellet.

33. The method of claim 1, wherein the fungus is Penicillium menonorum, wherein the fungal biomass is dried prior to combining with whey, wherein the fungal biomass comprises about 25% to 75% (cdw) crude protein, and wherein the admixture comprises between 80% and 95% fungal biomass by weight.

34. The method of claim 33, wherein said fungal biomass has been substantially depleted of a lipid product.

35. The method of claim 1, wherein said fungal biomass comprises a fatty acid.

36. The method of claim 35, wherein the fatty acid is selected from the group consisting of myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachic acid, behenic acid, lignoceric acid and combinations thereof.

37. The method of claim 36, wherein the fatty acid is selected from the group consisting of palmitic acid, oleic acid, and linoleic acid.

38. The method of claim 36, wherein the fatty acid is linolenic acid.

39. The method of claim 38, wherein the fatty acid is present as TAG, and at least 3.0% of the TAG is linolenic acid.