Method and system for measuring urea

• US 9,999,390 B2
• Filed: 10/17/2013
• Issued: 06/19/2018
• Est. Priority Date: 10/17/2013
• Status: Active Grant

• Current Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Original Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Inventors: Yao, Huanfen, Liu, Zenghe
• Primary Examiner(s): Natnithithadha, Navin
• Assistant Examiner(s): MESSERSMITH, ERIC J
• Application Number:
  US14/056,872
• Publication Number:
  US 20150112175A1
• Time in Patent Office: 1,706 Days
• CPC Class Codes: A61B 5/0004 characterised by the type of physiological signal transmitted
• CPC Class Codes: A61B 5/14507 specially adapted for measuring characteristics of body fluids other than blood devices for taking samples of body liquids A61B10/0045
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1477 non-invasive
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/6803 Head-worn items, e.g. helmets, masks, headphones or goggles
• CPC Class Codes: A61B 5/6821 Eye

Abstract

A body-mountable urea sensing device includes an electrochemical sensor embedded in a polymeric material configured for mounting to a surface of an eye. The electrochemical sensor includes a working electrode, a reference electrode, and a reagent localized near the working electrode that selectively reacts with urea. A potentiometric voltage between the working electrode and the reference electrode is related to a concentration of urea in a fluid to which the electrochemical sensor is exposed; the voltage is measured by the body-mountable device and wirelessly communicated.

Claims

1. A body-mountable device comprising:

a shaped polymeric material having a channel formed therein;

a substrate at least partially embedded within the shaped polymeric material and adjacent to the channel;

an antenna disposed on the substrate;

an electrochemical sensor disposed on the substrate and comprising;

a working electrode;

a protective layer comprising a polymer permeable to urea;

a reagent that selectively reacts with urea, wherein the reagent is localized within a reagent layer disposed in the channel between the protective layer and the working electrode; and

a reference electrode; and

a controller disposed on the substrate and embedded within the shaped polymeric material, wherein the controller is electrically connected to the electrochemical sensor and the antenna, wherein the controller is configured to;

(i) measure a potentiometric voltage between the working electrode and the reference electrode related to the concentration of urea in a fluid to which the body-mountable device is exposed; and

(iii) use the antenna to indicate the measured potentiometric voltage.

2. The body-mountable device according to claim 1, wherein the fluid is a tear fluid of an eye, wherein the shaped polymeric material has a concave surface and a convex surface, wherein the concave surface is configured to be removably mounted over a corneal surface and the convex surface is configured to be compatible with eyelid motion when the concave surface is so mounted.

3. The body-mountable device according to claim 1, wherein the reagent layer comprises a pH-responsive oxide.

4. The body-mountable device according to claim 3, wherein the pH-responsive oxide is in the form of a nano-powder, wherein the reagent that selectively reacts with urea is disposed on the particles of the nano-powder.

5. The body-mountable device according to claim 3, wherein the pH-responsive oxide includes zinc oxide.

6. The body-mountable device according to claim 3, wherein the pH-responsive oxide includes iridium oxide.

7. The body-mountable device according to claim 1, wherein the reagent comprises urease.

8. The body-mountable device according to claim 1, wherein the working electrode comprises platinum and the reference electrode comprises silver/silver-chloride.

9. The body-mountable device according to claim 1, wherein the antenna is configured to receive radio frequency energy to power the body-mountable device.

10. The body-mountable device according to claim 1, wherein using the antenna to indicate the measured potentiometric voltage comprises sending a radio frequency signal.

11. The body-mountable device according to claim 1, wherein using the antenna to indicate the measured potentiometric voltage comprises reflecting a radio frequency signal.

12. A method comprising:

exposing a working electrode and a reference electrode disposed in a body-mountable device to a fluid via a protective layer and a reagent layer disposed in a channel of the body-mountable device, wherein the protective layer comprises a polymer permeable to urea, wherein the reagent layer comprises a reagent that selectively reacts with urea, wherein the body-mountable device additionally includes an antenna and measurement electronics, and wherein the working electrode, reference electrode, antenna, and measurement electronics are disposed on a substrate that is at least partially embedded in a shaped polymeric material and adjacent to the channel;

measuring, by a controller, a potentiometric voltage between the working electrode and the reference electrode related to the concentration of urea in a fluid to which the body-mountable device is exposed, wherein the controller is disposed on the substrate and embedded within the shaped polymeric material; and

wirelessly indicating the measured voltage using the antenna.

13. The method of claim 12, wherein the fluid is a tear fluid of an eye, and wherein the shaped polymeric material has a concave surface and a convex surface, wherein the concave surface is configured to be removably mounted over a corneal surface of the eye and the convex surface is configured to be compatible with eyelid motion when the concave surface is so mounted.

14. The method of claim 12, wherein measuring the potentiometric voltage and wirelessly indicating the measured voltage using the antenna are performed in response to an interrogating radio frequency signal received by the antenna.

15. The method of claim 14, where the interrogating radio frequency signal is used to power the body-mountable device.

16. The method of claim 14, wherein wirelessly indicating the measured voltage comprises reflecting a version of the interrogating radio frequency signal.

17. The body-mountable device according to claim 12, wherein the reagent comprises urease.

18. The body-mountable device according to claim 12, wherein the reagent layer comprises zinc oxide.

19. The body-mountable device according to claim 12, wherein the reagent layer comprises iridium oxide.