Culture medium of adipose-derived stem cell, method for preparing the same, and composition including the same for promoting hair growth

• US 9,999,589 B2
• Filed: 08/29/2012
• Issued: 06/19/2018
• Est. Priority Date: 03/09/2012
• Status: Active Grant

• Current Assignee: Changwon National University Industry Academy Cooperation Corps
• Original Assignee: Changwon National University Industry Academy Cooperation Corps
• Inventors: Kim, Dong Wan, Seo, Mi Jung, Lee, Gwang, Joo, Woo Hong, Kim, Sun Hee
• Primary Examiner(s): Tate, Chris R
• Assistant Examiner(s): White, Douglas F
• Application Number:
  US14/384,044
• Publication Number:
  US 20150037435A1
• Time in Patent Office: 2,120 Days
• Field of Search: None
• CPC Class Codes: A61K 2800/70 Biological properties of the composition as a whole
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 8/981 of mammals or bird
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2500/84 from mammals
• CPC Class Codes: C12N 2506/1384 from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

Provided are a culture medium of an adipose-derived stem cell, a method for preparing the same, and a composition for promoting hair growth, in which the composition includes the culture medium. The adipose-derived stem cell (ADSC-T) according to the present invention exhibits long lifespan, improved cell proliferation rate, and extended proliferation period, as compared with a primary adipose-derived stem cell (ADSC), and thus, the adipose-derived stem cell (ADSC-T) can be usefully used for the study about the adipose-derived stem cell and the mass production of the culture medium of the adipose-derived stem cell. In addition, according to the present invention, the culture medium of the adipose-derived stem cell (ADSC-T) that expresses a T antigen of SV40 exhibits excellent hair growth effectiveness and can be usefully used as a raw material for the hair loss prevention and hair growing agents.

Claims

1. A method for promoting hair growth by administering to a subject in need thereof an effective amount of a pharmaceutical composition comprising a culture medium of an adipose-derived stem cell expressing a T antigen of SV40 (ADSC-T) as an active component.

2. A method for promoting hair growth by administering to a subject in need thereof an effective amount of a cosmetic composition comprising a culture medium of an adipose-derived stem cell expressing a T antigen of SV40 (ADSC-T) as an active component.