Cellular extracts

• US 9,999,639 B2
• Filed: 04/18/2016
• Issued: 06/19/2018
• Est. Priority Date: 05/11/2006
• Status: Active Grant

• Current Assignee: Regenics AS
• Original Assignee: Regenics AS
• Inventors: Gammelsaeter, Runhild, Remmereit, Jan
• Primary Examiner(s): Bertoglio, Valarie E
• Application Number:
  US15/131,198
• Publication Number:
  US 20160228475A1
• Time in Patent Office: 792 Days
• CPC Class Codes: A61K 2800/805 Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 35/65 Amphibians, e.g. toads, frogs, salamanders or newts
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61K 9/06 Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels composition of ointments, creams or gels A61K47/00
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/12 Keratolytics, e.g. wart or anti-corn preparations
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. The agents include compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products.

Claims

1. A method of making a composition suitable for topical administration from fish eggs comprising:

a) providing fish eggs;

b) processing said eggs to provide a cytoplasmic fraction from said eggs;

c) heating said cytoplasmic fraction to a temperature of from about 50°

C. to about 90°

C. for a period of greater than one minute to provide a heated cytoplasmic fraction; and

d) combining said heated cytoplasmic fraction with one or more pharmaceutically acceptable carriers to provide a composition suitable for topical administration selected from the group consisting of a cream, gel, emulsion, ointment, spray, powder and a lotion.

2. The method of claim 1, wherein said eggs are selected from the group consisting of fertilized and unfertilized eggs.

3. The method of claim 1, wherein said processing comprising homogenization of said eggs followed by centrifugation to separate lipids and solids from said cytoplasmic fraction.