Solid phase chromatographic immunoassay
First Claim
1. A test strip for determining the presence of an analyte in a liquid sample comprising a solid support, said solid support comprising at least a first portion and a second portion, said portions being in the same plane so as to permit capillary flow communication with each other;
- said first portion being the site for application of the liquid sample and further comprising a tracer site, said tracer site consisting of a tracer movably supported therein wherein said tracer comprises a ligand, which specifically binds to the analyte, conjugated to a visible particulate marker; and
said second portion being the site for visually determining the presence of the visible particulate marker, said second portion consisting of a binder immobilized therein which specifically binds to the analyte.
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Accused Products
Abstract
A chromatographic test strip comprising a solid support having at least a first portion and a second portion with said portions being in the same plane so as to permit capillary flow communication with each other. The sample is added to the first portion. The first portion also may comprise a tracer portion having a tracer movably supported therein. The tracer consists of a visible particulate marker. In the second portion, a binder is immobilized. The test strip is useful in a variety of immunoassays.
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Citations
32 Claims
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1. A test strip for determining the presence of an analyte in a liquid sample comprising a solid support, said solid support comprising at least a first portion and a second portion, said portions being in the same plane so as to permit capillary flow communication with each other;
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said first portion being the site for application of the liquid sample and further comprising a tracer site, said tracer site consisting of a tracer movably supported therein wherein said tracer comprises a ligand, which specifically binds to the analyte, conjugated to a visible particulate marker; and
said second portion being the site for visually determining the presence of the visible particulate marker, said second portion consisting of a binder immobilized therein which specifically binds to the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
a) adding a liquid sample to the first portion of the test strip of claim 1;
b) allowing sufficient time for the liquid sample to flow to the second portion of the test strip; and
c) determining the presence of the analyte in the liquid sample by visual inspection of the second portion for the visible particulate marker wherein the presence of the analyte is indicated by the presence of the visible particulate marker.
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10. The method of claim 9 wherein the liquid sample is added to the test strip by immersing the first portion into the liquid sample.
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11. A test strip for determining the amount of an analyte in a liquid sample comprising a solid support, said solid support comprising at least a first portion and a second portion, said portions being in the same plane so as to permit capillary flow communication with each other;
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said first portion being the site for application of the liquid sample and further comprising a tracer site, said tracer site consisting of a tracer movably supported therein wherein said tracer comprises a ligand, which is the analyte or an analog thereof, conjugated to a visible particulate marker; and
said second portion being the site for visually determining the amount of the visible particulate marker, said second portion consisting of a binder immobilized therein which specifically binds to the ligand. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
a) adding a liquid sample to the first portion of the test strip of claim 11;
b) allowing sufficient time for the liquid sample to flow to the second portion of the test strip; and
c) determining the presence of the analyte in the liquid sample by visual inspection of the second portion for the visible particulate marker wherein the presence of the analyte is indicated by the absence of the visible particulate marker.
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20. A no-wash, one-step method for determining the amount of an analyte in a liquid sample consisting of the steps of:
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a) adding a liquid sample to the first portion of the test strip of claim 12;
b) allowing sufficient time for the liquid sample to flow to the second portion and the third portions of the test strip; and
c) determining the amount of the analyte present in the liquid sample by visual inspection of the second portion and the third portion for the amount of the visible particulate marker bound in each portion wherein the presence of the analyte is indicated by the absence of the visible particulate marker.
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21. The method of claim 19 wherein the liquid sample is added to the test strip by immersing the first portion into the liquid sample.
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22. The method of claim 20 wherein the liquid sample is added to the test strip by immersing the first portion into the liquid sample.
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23. The test strip of claim 11 wherein the solid support comprises nitrocellulose.
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24. A method of detecting an analyte in a liquid sample, the method comprising the steps of:
(a) providing a solid support comprising;
- View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32)
Specification