Methods and apparatus for synthesizing labeled combinatorial chemistry libraries

• US RE39,545 E1
• Filed: 05/07/2004
• Issued: 04/03/2007
• Est. Priority Date: 02/02/1995
• Status: Expired due to Fees

• Current Assignee: NEXUS Biosystems, Inc. (Azenta, Inc.)
• Original Assignee: NEXUS Biosystems, Inc. (Azenta, Inc.)
• Inventors: Cargill, John
• Primary Examiner(s): Ponnaluri; Padmashri
• Application Number:
  US10/841,423
• Time in Patent Office: 1,061 Days
• Field of Search: 436/518, 436/523, 436/524, 436/525, 436/526, 436/527, 436/528, 235/439, 530/334, 530/335, 435/6, 435/DIG.49, 435/DIG.34, 435/DIG.1, 435/DIG.9, 435/DIG.21, 435/DIG.29
• US Class Current: 436/518
• CPC Class Codes: N/A

Abstract

The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.

Claims

1. A pre-encoded substrate useful for being linked to a single oligomer structure whereby said pre-encoded substrate bears a unique identifier tag, said tag being a pre-encoded microchip identifying said oligomer structure.

3. One or a plurality of the pre-encoded substrate of claim 1 useful for in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:

a) apportioning said pre-encoded substrates among a plurality of reaction vessels;

b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;

c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;

d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and

e) repeating steps a) through c) from at least one to about twenty times wherein said preencoded substrate being a pre-encoded microchip.

5. One or a plurality of the pre-encoded substrate of claim 1 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:

a) apportioning said pre-encoded substrates among a plurality of reaction vessels;

b) exposing said pre-encoded substrates in each reaction vessels to one or a plurality of monomers;

c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;

d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and

e) repeating steps a) through c) from at least one to about twenty times wherein said pre-encoded substrate being a pre-encoded microchip.

2. An encodable substrate useful for being linked to a single oligomer structure whereby said encodable substrate has a unique identifier tag, said tag being a encodable microchip identifying said oligomer structure.

4. One or a plurality of the encodable substrate of claim 2 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:

a) apportioning said encodable substrates among a plurality of reaction vessels;

b) exposing said encodable substrates in each reaction vessels to one or a plurality of transformation events;

c) adding first identifier information to said encodable substrates;

d) apportioning said encodable substrates among a plurality of reaction vessels; and

e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.

6. One or a plurality of the encodable substrate of claim 2 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:

a) apportioning said encodable substrates among a plurality of reaction vessels;

b) exposing said encodable substrates in each reaction vessels to one or a plurality of monomers;

c) adding first identifier information to said encodable substrates;

d) apportioning said encodable substrates among a plurality of reaction vessels; and

e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.