Methods and apparatus for synthesizing labeled combinatorial chemistry libraries

• US RE39,571 E1
• Filed: 05/07/2004
• Issued: 04/17/2007
• Est. Priority Date: 02/02/1995
• Status: Expired due to Fees

• Current Assignee: NEXUS Biosystems, Inc. (Azenta, Inc.)
• Original Assignee: NEXUS Biosystems, Inc. (Azenta, Inc.)
• Inventors: Cargill, John
• Primary Examiner(s): Ponnaluri; Padmashri
• Application Number:
  US10/841,421
• Time in Patent Office: 1,075 Days
• Field of Search: 435/6, 435/287.1, 435/DIG.1, 435/DIG.14, 435/DIG.21, 435/DIG.34, 435/DIG.49, 435/DIG.9, 435/DIG.29, 436/518, 436/523, 436/524, 436/525, 436/526, 436/527, 436/528, 536/24.3, 422/68.1, 369/13.01, 530/334
• US Class Current: 436/518
• CPC Class Codes: N/A

Abstract

The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.

Claims

1. A device for tracking synthesis of a library member of a combinatorial library, the device comprising:

a synthesis support having a surface adapted for linking to a first monomer;

an identifier tag disposed within or linked to the synthesis support, the identifier tag comprising encoded information readable by a radiofrequency scanner or bar-code scanner that is unique to the synthesis support and means for scanning the identifier tag, wherein, when a first monomer is linked to the synthesis support, the first monomer and any oligomer synthesized using the first monomer is identified.

2. The device of claim 1 wherein the identifier tag comprises a microchip having an electronic memory within which is stored unique electronic information.

3. The device of claim 2 wherein the microchip comprises means for receiving and transmitting a radiofrequency signal.

4. The device of claim 2, wherein the microchip is encodable for recording synthesis information during synthesis of the library member.

5. The device of claim 4, wherein the synthesis information comprises descriptions of synthesis steps.

6. The device of claim 2, wherein the microchip is pre-encoded with an identification of the synthesis support.

7. The device of claim 2, wherein the microchip is encased in a glass or polymeric material.

8. The device of claim 7, wherein the polymeric material is derivatized with a functional group or linker.

9. The device of claim 8, wherein the polymeric material is selected from the group consisting of polystyrene-divinyl benzene, polyethylene-grafted polystyrene, polyacrylamide kieselguhr composite, and controlled pore glass.

10. The device of claim 8, wherein the linker is cleavable by a reaction selected from the group consisting of thermal, photochemical, electrochemical, oxidative, and reductive reactions.

11. The device of claim 3, wherein the microchip is powered by the radiofrequency signal.

12. The device of claim 2, wherein the unique electronic information is digitally encoded.

13. The device of claim 2, wherein the synthesis support is in a form selected from the group consisting of pellets, disks, capillaries, hollow fibers, solid fibers, cellulose beads, pore-glass beads, silica gels, polystyrene beads, grafted co-poly beads, poly-acrylimide beads, latex beads, dimethylacrylamide beads, and glass particles.

14. The device of claim 2, wherein the first monomer is linked to the synthesis support by a linker comprising a spacer residue.

15. The device of claim 1, wherein the identifier tag comprises a bar code having unique information.

16. The device of claim 15, wherein the bar code is capable of receiving sequential synthesis information.

17. The device in claim 16, wherein the synthesis information comprises descriptions of synthesis steps.

18. The device of claim 15, wherein the barcode is pre-encoded with the identity of the synthesis support.

19. The device of claim 15, wherein the barcode is encased in a glass or polymeric material.

20. The device of claim 19, wherein the polymeric material is derivatized with a functional group or linker.

21. The device of claim 20, wherein the polymeric material is selected from the group consisting of polystyrene-divinyl benzene, polyethelene-grafted polystyrene, polyacrylamide kieselguhr composite, and controlled pore glass.

22. The device of claim 20, wherein the linker is cleavable by a reaction selected from the group consisting of thermal, photochemical, electrochemical, oxidative, and reductive reactions.