Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
First Claim
1. A labeled synthetic oligomer library comprising a plurality of different members each member comprising an oligomer linked to an identifier which is a microchip encoded with information identifying the structure of said oligomer.
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Abstract
The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.
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Citations
49 Claims
- 1. A labeled synthetic oligomer library comprising a plurality of different members each member comprising an oligomer linked to an identifier which is a microchip encoded with information identifying the structure of said oligomer.
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10. A labeled synthetic oligomer library produced by synthesizing on each of a plurality of pre-encoded substrates, each of which has a unique identifier tag, which is a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
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a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (11, 12, 13, 14, 15)
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16. A labeled synthetic oligomer library produced by synthesizing on each of a plurality of encodable substrates each of which contains a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
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a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessel to one or a plurality of transformation events;
c) adding identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23)
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24. A method of preparing a labelled labeled synthetic oligomer library comprising a plurality of different members, each member comprising a pre-encoded substrate linked to a single oligomer structure and bearing a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
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a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (26, 29)
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25. A method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising an encodable substrate linked to a single oligomer structure and having a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
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a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessel to one or a plurality of transformation events;
c) adding first identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (27, 28)
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30. A labeled synthetic oligomer library produced by synthesizing on each of a plurality of pre-encoded substrates, each of which has a unique identifier tag which is a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
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a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of monomers;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (31, 32, 33, 34, 35)
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36. A labeled synthetic oligomer library produced by synthesizing on each of a plurality of encodable substrates each of which has a unique identifier tag which is a microchip encodable with information, a single oligomer structure obtained by a method comprising the steps of:
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a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessel to one or a plurality of monomers;
c) adding identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43)
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44. A method of preparing a labelled labeled synthetic oligomer library comprising a plurality of different members, each member comprising a pre-encoded substrate linked to a single oligomer structure and bearing a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
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a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of monomers;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (48)
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45. A method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising an encodable substrate linked to a single oligomer structure and having a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
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a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessel to one or a plurality of monomers;
c) adding first identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times. - View Dependent Claims (46, 47, 49)
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Specification